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目的初步了解广东省水产品中香港海鸥型菌(Laribacter hongkongensis)污染及分离株的耐药情况。方法采用改良头孢哌酮麦康凯琼脂(CMA)作为分离培养基,以API 20NE鉴定系统鉴定结果作为确认依据;采用K-B法进行药物敏感试验。结果222份水产样本检出15株目标菌,阳性率6.76%。其中14株分离自草鱼肠脏,1株来自鲤鱼肠脏。分离株及香港海鸥型菌模式株HKU1的药敏结果,对β-内酰胺类抗生素如氨苄西林、头孢噻酚、头孢他啶、头孢曲松等均出现耐药株,未见耐庆大霉素和阿米卡星株。结论结果表明香港海鸥型菌在广东省的草鱼污染率最高,为17.3%(14/81),分离株对β-内酰胺类抗生素耐药情况较严重。
Objective To understand the pollution of Laribacter hongkongensis and the drug resistance of isolated strains in Guangdong aquatic products. Methods The modified cefoperazone-MacConkey agar (CMA) was used as the separation medium, and the API 20NE identification system was used as the confirmation basis. K-B method was used for drug sensitivity test. Results 222 strains of aquatic samples were detected 15 target bacteria, the positive rate of 6.76%. Among them, 14 were isolated from grass carp intestinal gut and 1 from carp intestinal gut. Isolates and Hong Kong seagull model strain HKU1 susceptibility results, β-lactam antibiotics such as ampicillin, ceftibucin, ceftazidime, ceftriaxone, etc. were resistant strains, no resistance to gentamicin and Amikacin strain. Conclusion The results showed that the highest pollution rate of seagull in Hong Kong was 17.3% (14/81). The isolates were more resistant to β-lactam antibiotics.