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目的 :研究白细胞介素 2 (IL 2 )对人乳腺癌Bcap 37细胞系增殖的影响 ,并探讨其可能的机制。方法 :以培养的Bcap 37细胞进行了以下实验 :用MTT比色法 ,测定IL 2对该细胞增殖的剂量效应 ;用免疫组化染色法 ,检测其表面IL 2的表达 ;用RT PCR技术 ,检测该细胞中IL 2Rα、β和γmRNA的表达 ;用流式细胞术 ,观察IL 2对该细胞细胞周期各阶段DNA含量的影响 ;用激光扫描共聚焦显微镜技术 ,检测IL 2对该细胞内Ca2 + 水平的影响。结果 :IL 2 (1× 10 5～ 1× 10 6U/L)可显著促进乳腺癌细胞Bcap 37的增殖。培养的Bcap 37细胞可分泌IL 2并表达IL 2Rα、β和γ。用流式细胞仪检测Bcap 37细胞中DNA的含量发现 ,IL 2 (1× 10 5U/L)可提高该细胞由G1期进入G2 和S期的比例。激光共聚焦法检测到IL 2 (1× 10 5U/L)可使Bcap 37细胞内的Ca2 + 浓度降低。结论 :IL 2参与了对乳腺癌细胞增殖的调节 ,其促增殖作用与该细胞上IL 2受体的表达 ,细胞中DNA含量的变化和Ca2 + 浓度的降低有关。
Objective: To investigate the effect of interleukin 2 (IL 2) on the proliferation of human breast cancer Bcap 37 cell line and to explore its possible mechanism. Methods: Bcap 37 cells cultured in the following experiments: using MTT colorimetric assay IL 2 dose-dependent proliferation of the cells; using immunohistochemical staining to detect the surface of IL 2 expression; using RT PCR technology, The expression of IL 2Rα, β and γmRNA in this cell line was detected by flow cytometry. The effect of IL 2 on the DNA content of the cell cycle was observed by laser scanning confocal microscopy. + Level of influence. Results: IL 2 (1 × 10 5 ~ 1 × 10 6 U / L) could significantly promote the proliferation of Bcap 37 breast cancer cells. The cultured Bcap 37 cells can secrete IL 2 and express IL 2Rα, β and γ. Using flow cytometry to detect DNA content in Bcap 37 cells, it was found that IL 2 (1 × 10 5 U / L) increased the proportion of cells entering G2 and S phase from G1 phase. IL-2 (1 × 10 5 U / L) was detected by laser scanning confocal microscopy and the concentration of Ca 2+ in Bcap 37 cells was decreased. CONCLUSION: IL-2 is involved in the regulation of breast cancer cell proliferation and its role in promoting proliferation is related to the expression of IL-2 receptor, DNA content in cells and the decrease of Ca2 + concentration.