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目的 :研究中华眼镜蛇毒组分C(FractionCfromNajaNajaActraVenom ,FC)体外对人肝癌细胞株的细胞毒作用和机理 ,并与传统肝动脉插管栓塞 灌注化疗 (TranscatheterArterialChemoembolization ,TACE)药进行比较。方法 :应用MTT法、流式细胞仪等方法 ,观察FC对人肝癌细胞株 (Bel 74 0 2 )、人支气管上皮细胞株 (HBE16 )及人羊膜细胞株 (HAM )的细胞毒作用、量效关系、凋亡率等 ,并与 5 氟尿嘧啶 (5 Fu)、丝裂霉素 (MMC)、阿霉素 (ADM )等对上述细胞的细胞毒作用进行比较。结果 :FC对人肝癌细胞有明显的抑制作用 ,其 2 4及 4 8h的IC50 分别为 4 .34和 3.96 μg ml,且呈良好的量效关系 ,而正常人支气管上皮细胞 (HBE16 )的IC50 则分别高达10 4 6和 11.82 μg ml,两细胞株间存在差异显著性 (P <0 .0 1)。FC还能诱导肝癌细胞凋亡 ,凋亡率随浓度升高而增加。结论 :在本实验中 ,FC能有效抑制肝癌细胞株的生长 ,抑制作用与浓度呈正相关 ,且能诱导肝癌细胞凋亡 ,与传统TACE化疗药相比较 ,FC是有效的、能选择性抑制肝癌细胞生长的新型抗癌药物。
OBJECTIVE: To study the cytotoxicity and mechanism of Fraction C from NajaNaja ActVenom (FC) on human hepatoma cell lines in vitro and to compare with traditional transcatheter arterial chemoembolization (TACE). Methods: The cytotoxic effects of FC on Bel 7402 cells, human bronchial epithelial cells (HBE16) and human amniotic membrane cells (HAM) were observed by MTT assay and flow cytometry. And the apoptosis rates were compared with those of 5 Fu, MMC and ADM. Results FC showed obvious inhibitory effect on human hepatocellular carcinoma cells, with IC50 values of 4.34 and 3.96 μg ml at 24 and 48 h, respectively, showing a good dose-response relationship, whereas IC50 values of normal human bronchial epithelial cells (HBE16) Respectively, up to 1046 and 11.82 μg ml, there was a significant difference between the two cell lines (P <0.01). FC can also induce apoptosis of hepatocellular carcinoma cells, the apoptosis rate increases with increasing concentration. Conclusion: In this experiment, FC can effectively inhibit the growth of hepatocellular carcinoma cell lines, the inhibition is positively correlated with the concentration, and can induce the apoptosis of hepatoma cells. Compared with the traditional TACE chemotherapeutic agents, FC is effective and can selectively inhibit liver cancer Cell growth of new anti-cancer drugs.