论文部分内容阅读
近年来,绿脓杆菌已被认为是医院感染中最常见的病原菌之一.它广泛的存在于自然界,可以通过交叉感染、环境污染、医源性感染、内源性感染等方式造成院内感染.我们于1995年对江苏省人民医院烧伤整形病区绿脓杆菌污染情况进行了调查,报告如下:1 材料与方法1.1 采样对象 医生、护士、工人的鼻腔、手、白大衣、裤、鞋;病人的鼻腔、手、肛门、创面;陪护人员的鼻腔、手;病区环境、医疗器械和用具等.1.2 培养基①萘叮酮酸培养基(NAC);②乙酰胺选择培养基;③普通营养肉汤及琼脂培养基.1.3 噬菌体及其分型方法 所用24株绿脓杆菌噬菌体由作者张晋琳等制备,按“绿脓假单胞菌噬菌体分型(江苏噬研法)”程序进行分型.1.4 采样与分离鉴定 采样重复两次,间隔15天.将灭菌棉签在生理盐水中浸湿后采样,然后放置NAC液体培养基试管中,于42℃增菌培养,翌日见管内有混浊者,均转种NAC琼脂平板于37℃培养过夜,分离单个菌落.菌株能产生色素、乙酰胺酶试验阳性、使硝
In recent years, Pseudomonas aeruginosa has been considered as one of the most common pathogenic bacteria in nosocomial infections.It widely exists in nature and can cause nosocomial infection through cross-infection, environmental pollution, iatrogenic infection and endogenous infection. We in 1995, Jiangsu Provincial People’s Hospital burn plastic surgery ward Pseudomonas aeruginosa pollution were investigated, the report is as follows: 1 Materials and methods 1.1 Sampling objects doctors, nurses, workers nose, hand, white coat, pants, shoes; patients Nasal cavity, hands, anus and wounds; nasal cavity and hands of entourage; ward environment, medical equipment and utensils, etc. 1.2 Media ① Naphtoluronic acid medium (NAC); ② Acetamide selective medium; ③ Ordinary nutrition Broth and agar medium.1.3 Phage and its typing method 24 strains of Pseudomonas aeruginosa phage prepared by the author Zhang Jinlin, etc., according to “Pseudomonas aeruginosa phage classification (Jiangsu pharyngeal research method)” program for typing. 1.4 Sampling and separation identification Sampling repeated two intervals of 15 days.The sterilized cotton swabs were immersed in saline after sampling, and then placed in NAC liquid culture tube, cultured at 42 ℃ enrichment, see the tube the next day with mixed Turbidity, were transplanted NAC agar plate overnight at 37 ℃, the separation of individual colonies strains can produce pigment, acetamidase test positive, so that nitrate