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目的 :以RT -PCR技术测定和分析有氧运动干预对饮食性高胆固醇血症大鼠肝脏高密度脂蛋白受体 (SR -BⅠ )基因表达的影响 ,进一步探讨有氧运动对胆固醇逆向转运 (RCT)通路影响的分子机理。方法 :雄性SD大鼠 40只 ,随机分为 :(1 ) 8周普通膳食对照组 (NS ,n =1 0 ) ,(2 ) 8周高脂膳食对照组 (HS ,n =1 0 ) ,(3 ) 8周普通膳食 +运动组(NE ,n=1 0 )和 (4 ) 8周高脂膳食 +运动组(HE ,n =1 0 )。用RT -PCR方法测定肝脏SR -BⅠmRNA表达。结果 :(1 )NE组大鼠肝脏SR -BⅠmRNA的表达较NS组无显著性变化 ;(2 )HS组SR -BⅠmRNA的水平显著低于NS组 (P <0 0 1 ) ;(3 )HE组SR -BⅠmRNA较HS组显著升高 (P <0 0 1 ) ,但是仍显著低于NS组 (P <0 0 1 )。结论 :有氧运动可在转录水平纠正高脂负荷下调大鼠肝脏SR -BⅠmRNA表达的作用 ,这一分子水平的调节有助于高脂负荷时的胆固醇逆向转运过程
OBJECTIVE: To determine the effect of aerobic exercise on the gene expression of hepatic high density lipoprotein receptor (SR-BⅠ) in rats with diet-induced hypercholesterolemia by RT-PCR and to further explore the effect of aerobic exercise on cholesterol reverse transport RCT) pathway. Methods: Forty male Sprague-Dawley rats were randomly divided into: (1) 8-week normal diet control group (NS, n = 10), (2) 8-week high fat diet control group (3) 8-week normal diet + exercise group (NE, n = 10) and (4) 8 week high-fat diet + exercise group (HE, n = 10). Liver RT-PCR method was used to determine SR-ⅠmRNA expression. (2) The level of SR-B mRNA in HS group was significantly lower than that in NS group (P <0.01); (3) The expression of SR-ⅠmRNA in liver of NE group was significantly lower than that in NS group Compared with HS group, SR-B mRNA expression was significantly increased (P <0.01), but still significantly lower than that in NS group (P <0.01). CONCLUSION: Aerobic exercise can regulate the expression of SR-B mRNA in rat liver at the transcriptional level by correcting the high-fat load. The regulation of this molecular level contributes to the reverse cholesterol transport process during high-fat loading