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目的 :探讨电刺激迷走神经对兔内毒素(LPS)血症肝脏损害的作用。方法 :16只兔随机分为实验组(A组 )和对照组 (B组 )。切断双侧颈迷走神经干 ,按 10 0μg kg静注大肠杆菌LPS ,A组选择左迷走神经近心端接刺激电极 ,在注射LPS前后各持续电刺激 (电压 10V ,频率 5Hz ,波宽 5ms) 10min ,共 2次。分别于输注LPS前及后 30、6 0、12 0 180 2 4 0、30 0min检测 2组血清中丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST)、肿瘤坏死因子 α(TNF α)及IL 10含量 ,并于实验结束后取肝标本进行病理检查。结果 :静注LPS后 ,与B组比较 ,A组血清中ALT ,AST和TNF α显著降低 ,而IL 10水平明显升高 ,肝脏炎性病理改变明显减轻。结论 :电刺激兔迷走神经传出纤维能抑制LPS导致的肝功能损害和炎症递质的产生 ,但并不抑制抗炎因子 ,从而减轻肝脏炎症 ,对肝功能有一定的保护作用。
Objective: To investigate the effect of electrical stimulation of the vagus nerve on liver damage induced by endotoxin (LPS) in rabbits. Methods: Sixteen rabbits were randomly divided into experimental group (A group) and control group (B group). The vagus nerve of the bilateral cervical spine was cut off, and the LPS of 10 0 μg kg was injected intravenously. In the A group, the left vagal nerve proximal cardiac stimulation electrode was selected and the electrical stimulation (voltage 10V, frequency 5Hz, wave width 5ms) A total of 2 times. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor alpha (TNF) and tumor necrosis factor alpha (TNF) were measured before and after LPS infusion at 30, 60, 120, 180, 240, α) and IL 10 content, and liver specimens were taken for pathological examination after the experiment. Results: Compared with group B, the serum levels of ALT, AST and TNFα in group A were significantly decreased, while the level of IL 10 was significantly increased in group A, and the pathological changes in liver were significantly reduced. CONCLUSION: Electrical stimulation of vagal nerve fibers in rabbit vagus can inhibit hepatic dysfunction and inflammatory mediators induced by LPS, but not inhibit anti-inflammatory cytokines, thereby reducing hepatic inflammation and protecting liver function.