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在构建了再生性外周神经组织cDNA文库的基础上，人工合成了睫状神经节神经营养因子（CNTF）阅读框架的引物，用PCR地高辛标记法标记了CNTF探针．筛选CDNA文库，得到了CNTF的阳性克隆，运用PCR法证实了克隆中存在着全长CNTF阅读框架，Sanger法测定了DNA序列，证实序列无误。CNTF基因克隆为从基因水平上研究CNTF的分子生物学作用机制打下基础．
Based on the constructed cDNA library of regenerative peripheral nerve tissue, primers of ciliary ganglion neurotrophic factor (CNTF) reading frame were synthesized, and CNTF probe was labeled with PCR digoxigenin method. The CDNA library was screened and the positive clones of CNTF were obtained. The full-length CNTF reading frame was confirmed by PCR, the DNA sequence was determined by Sanger method, and the correct sequence was confirmed. The cloning of CNTF gene lays the foundation for studying the molecular biological mechanism of CNTF at the gene level.