论文部分内容阅读
放线菌素23-21(Act23-21)对人早幼粒白血病细胞(HL-60)具有杀伤和诱导分化双重作用,其效应的强度和性质对剂量变化较敏感。采用Act23-21 5-50ng/ml处理24～72h,HL-60细胞的PC值有不同程度的降低,可低于10％,以集落形成法测得剂量—细胞存活率曲线为阈值指数型,杀伤指数达2以上。Act23-21在低浓度下(1.0～4.0ng/ml)能诱导HL-60细胞发生形态学和功能学分化,成熟细胞可达49％,细胞的分化性状能持续表达。Act23-21的诱导作用比放线菌素D(Act D)稍强,逊于二甲基亚砜(DMSO),而其诱导细胞分化性状持续表达的能力强于DMSO。
Act23-21 acts both in killing and inducing differentiation of human promyelocytic leukemia cells (HL-60), the intensity and nature of which is sensitive to dose changes. With Act23-21 5-50ng / ml for 24-72h, the PC value of HL-60 cells decreased to some extent, which could be lower than 10%. The curve of dose-cell survival rate was the threshold exponential curve measured by colony- Kill index of 2 or more. Act23-21 induced morphological and functional differentiation of HL-60 cells at low concentration (1.0-4.0ng / ml), with 49% of mature cells and continuous differentiation of cells. Act23-21 was slightly stronger than Act D and lower than DMSO. The ability of Act23-21 to induce continuous differentiation of cell differentiation was stronger than that of DMSO.