论文部分内容阅读
目的:研究尖山橙和龙州山橙中的抗肿瘤生物碱成分方法:采用层析柱和制备高效液相进行化合物分离,运用1D、2D-NMR及MS等波谱分析技术鉴定化合物结构。采用MTT法进行人体肿瘤细胞毒体外活性实验。结果:分离得到两个新的双吲哚生物碱melofusine I (1)和melomorsine I (2),以及22个已知吲哚生物碱,测试了24个化合物对5种(SW480,SMMC-7721,HL-60,MCF-7,A-549)人体肿瘤细胞的细胞毒活性。结论:化合物1-2是新的双吲哚生物碱,化合物tabersonine(3) and 19S-vindolinine(14)具有抑制5种人体肿瘤细胞株的活性。
OBJECTIVE: To study the antitumor alkaloid constituents of Jianshan orange and Longzhou orange. Methods: The compounds were separated by column and preparative high performance liquid chromatography. The structures of compounds were identified by 1D, 2D-NMR and MS spectral analysis. MTT assay was used to determine the in vitro cytotoxic activity of human tumor cells. Results: Two new indole alkaloids, melofusine I (1) and melomorsine I (2), and 22 known indole alkaloids were isolated and tested. The effects of 24 compounds on 5 (SW480, SMMC-7721, HL-60, MCF-7, A-549) human tumor cells. CONCLUSION: Compound 1-2 is a novel bisindole alkaloid. Compounds tabersonine (3) and 19S-vindolinine (14) inhibit the activity of 5 human tumor cell lines.