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以茶树子叶为外植体,研究茶树体细胞胚产生方式及形态。结果表明:茶树体细胞胚在子叶柄处直接发生,或产生胚性愈伤组织间接发生。体细胞胚间接发生途径中,子叶表面隆起是发生脱分化的标志,随后细胞团膨大、颜色变绿转化成胚性愈伤组织,其表面分化出半透明的颗粒状突起即为体细胞胚。继代培养中,体细胞胚的发生、萌发以及次级胚性愈伤组织的产生同时进行。体细胞胚的石蜡切片表明,此处细胞小且排列紧密,细胞质浓,有若干个分生区域,具有分生能力。
Tea cotyledon explants were used to study the production and morphology of somatic embryos in tea plants. The results showed that the somatic embryos of tea plants occurred directly in the cotyledonary petiole, or the embryogenic callus occurred indirectly. Somatic embryo indirect pathways, cotyledon surface protuberance is a sign of dedifferentiation, followed by cell mass expansion, green color into embryogenic callus, the surface of the differentiation of translucent granular protrusions that is somatic embryos. Subculture, the occurrence of somatic embryos, germination and secondary embryogenic callus simultaneously. Paraffin sections of somatic embryos indicate that the cells are small and closely arranged here, with dense cytoplasm and several meristematic regions with meristematic ability.