论文部分内容阅读
目的：探索ＳｉＯ２刺激ＴＨＰ－１细胞（具有肺泡巨噬细胞特性的人血单核细胞株）上清液是否可引起大鼠的矽肺样病变。方法：用ＳｉＯ２刺激ＴＨＰ－１细胞培养上清液经非暴露式气管内注入，０．５ｍｌ／次，１次／周，共４次，末次注射后１周处死，取肺脏常规切片，Ｈ．Ｅ染色，镜下观察病理变化，并通过５个系列浓度（０、５０、１００、２００和５００μｇ／ｍｌ）的ＳｉＯ２刺激ＴＨＰ－１细胞培养上清液作用于ＣＨＬ细胞（中国仓鼠肺成纤维细胞），采用四唑盐（ＭＴＴ）比色法检测ＣＨＬ细胞的增殖。结果：ＳｉＯ２刺激ＴＨＰ－１细胞培养上清液可引起大鼠肺内早期矽结节样病变。经ＭＴＴ分析，ＣＨＬ细胞增殖活性随着ＳｉＯ２剂量的增大而逐渐增强，呈剂量－效应关系。结论：ＳｉＯ２刺激的ＴＨＰ－１细胞上清液在体内可引起早期矽结节样病变，在体外可以引起肺成纤维细胞增殖
AIM: To investigate whether SiO2-stimulated supernatant of THP-1 cells (human blood mononuclear cell line with alveolar macrophages) can cause silicosis in rats. Methods: THP-1 cell culture supernatants were infused with non-exposed endotracheal intratracheal instillation of 0.5ml / time, once a week for 4 times, and sacrificed one week after the last injection. E staining. Pathological changes were observed microscopically. THP-1 cell culture supernatant was stimulated by 5 series of SiO2 (0, 50, 100, 200 and 500μg / ml) to induce apoptosis in CHL cells (Chinese hamster lung fibroblasts ), The proliferation of CHL cells was detected by MTT assay. RESULTS: Silica-stimulated THP-1 cell culture supernatant could induce early silicotic nodular lesions in the lungs of rats. MTT assay showed that the proliferation activity of CHL cells gradually increased with the increase of the dose of SiO2, showing a dose-response relationship. CONCLUSIONS: SiO2-stimulated THP-1 cell supernatants can cause early silicotic nodular lesions in vivo and can cause pulmonary fibroblast proliferation in vitro