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目的：探讨表面分子在自然杀伤（ＮＫ）细胞活化中的信号分子作用及其机制。方法：采用多种粘附分子单抗及配体刺激ＮＫ细胞，ＥＬＩＳＡ法定量检测刺激后ＮＫ细胞释放的γ干扰素（ＩＦＮ－γ）作为ＮＫ细胞活化指标，观察各种粘附分子在ＮＫ细胞释放ＩＦＮ－γ中的信号作用。结果：单一ＣＤ４５单抗刺激即可使ＮＫ细胞活化；两种分别为ＩｇＧ１，ＩｇＧ２ａ亚类的ＣＤ４５单抗及ＩｇＧ２ａ亚类ＣＤ４５单抗的Ｆ（ａｂ）２均体现对ＮＫ细胞的独立刺激作用。结论：ＣＤ４５分子介导的ＮＫ细胞活化与ＣＤ１６－Ｆｃ段间相互作用无关，ＣＤ４５分子交联是ＮＫ细胞活化的独立刺激信号
OBJECTIVE: To investigate the role of surface molecules in the activation of natural killer (NK) cells and its mechanism. Methods: NK cells were stimulated by a variety of adherent monoclonal antibodies and ligands, and the IFN-γ released by NK cells was detected quantitatively by ELISA as the NK cell activation index. The effects of various adhesion molecules on NK cells Release of IFN-γ in signaling. Results: NK cells were activated by stimulation with a single anti-CD45 monoclonal antibody. F (ab) 2, two anti-CD45 monoclonal antibodies of IgG1 and IgG2a subclasses and CD45 monoclonal antibodies of IgG2a subtype, respectively, showed independent stimulation of NK cells . CONCLUSION: The activation of NK cells mediated by CD45 molecules has nothing to do with the interaction between CD16-Fc segments. The CD45 molecule cross-linking is an independent stimulatory signal of NK cell activation