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1 对象与方法1.1 对象 本院健康职工及献血员20名,年龄20～40岁,受试前2W内未服任何影响血小板的药物及食品。1.2 仪器及试剂 仪器Chrono-Log 560型全血小板聚集仪(Chrono-Log公司、美国);Bio-Rod Medel 450型酶标仪(瑞典);ZXN-A型自转式血小板粘附仪(中国医科大学仪器厂)。试剂包被鼠(IgG-Coat)、标准IgG(IgG-ST)、羊抗鼠酶标二抗
1 Subjects and Methods 1.1 Subjects There are 20 health workers and blood donors in this hospital, aged 20 to 40 years old. No drugs or foods affecting platelets were administered within 2 W before the test. 1.2 Instruments and Reagents Instruments Chrono-Log 560 Total Platelet Aggregator (Chrono-Log, USA); Bio-Rod Medel 450 Microplate Reader (Sweden); ZXN-A Autorotation Platelet Adhesion Instrument (China Medical University) Instrument factory). Reagent-coated mouse (IgG-Coat), standard IgG (IgG-ST), goat anti-mouse enzyme-conjugated secondary antibody