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目的探讨正常妊娠女性及流产患者外周血及绒毛组织激活素结合蛋白(FS)水平变化。方法采用酶联免疫吸附试验(ELISA)检测外周血FS水平,实时定量PCR检测绒毛组织FS mRNA表达水平。结果正常妊娠女性外周血FS水平变化随孕期增加而增加;流产患者外周血FS水平明显低于正常早期妊娠女性外周血FS水平,统计学比较差异显著,P<0.01;流产患者绒毛组织FS mRNA表达水平也明显低于正常早期妊娠女性绒毛组织FS mRNA表达水平,统计学比较差异显著,P<0.01。结论流产患者外周血FS水平明显降低,检测外周血FS水平变化可能有助于流产诊断。
Objective To investigate the changes of peripheral blood and villus activin-binding protein (FS) levels in normal pregnant women and abortion patients. Methods Peripheral blood FS levels were detected by enzyme-linked immunosorbent assay (ELISA). The expression of FS mRNA in villi was detected by real-time quantitative PCR. Results The level of FS in peripheral blood of normal pregnant women increased with the increase of gestational age. The level of FS in peripheral blood of abortion patients was significantly lower than that of normal pregnant women, the difference was statistically significant (P <0.01). FS mRNA expression in villi The level of FS mRNA expression was also significantly lower than that of normal early pregnancy female chorion tissue, the difference was statistically significant (P <0.01). Conclusion The level of FS in peripheral blood of patients with abortion is significantly lower. Detecting the change of FS level in peripheral blood may contribute to the diagnosis of abortion.