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利用基因重组技术，将tpAcDNA与逆转录病毒载体LNSX重组构成LNS－ipA，转染病毒包装细胞，形成的重组逆转录病毒颗粒再用来感染牛血管内皮细胞。经G418筛选后的单克隆细胞培液，分别经纤维蛋白板法和发包底物法测取活性。结果：经基因转染的PA317细胞和牛血管内皮细胞，在含人纤维蛋白原和凝血酶的纤维蛋白板上出现溶圈；随培养时间的延长，细胞数量增加，tpA分泌活性也增加。结论：含人tpAcDNA转录单位的牛血管内皮细胞，其纤溶活性明显增高。该研究为将来进行基因治疗奠定了基础。
Using recombinant DNA technology, tpA cDNA was recombined with retroviral vector LNSX to form LNS-ipA, which was transfected into virus packaging cells. The recombinant retrovirus particles were then used to infect bovine vascular endothelial cells. The monoclonal cell culture liquid screened by G418 was measured by fibrin plate method and contralateral substrate method respectively. Results: PA317 cells and bovine vascular endothelial cells transfected with the gene were dissolved in fibrin plate containing human fibrinogen and thrombin. With the prolongation of culture time, the number of cells and the secretion activity of tpA also increased. Conclusion: The fibrinolytic activity of bovine vascular endothelial cells containing human tpAcDNA transcripts is obviously increased. The research laid the foundation for future gene therapy.