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荧光原位杂交(FISH)技术以其简便、快捷、高敏感度及高特异度的优点,可一次检测成千上万的精子染色体而被广泛应用,并逐渐成为精子染色体检测的主要方法。许多研究用FISH技术检测男性的精子染色体发现,有毒物接触史、肿瘤患者放化疗后、染色体平衡易位患者及不育男性的非整倍体率较正常男性明显增加。特别是随着卵母细胞胞浆内单精子注射(ICSI)的应用,FISH在检测不育男性精子染色体中的作用更加明显。综述近年FISH在研究精子染色体非整倍体方面的进展。
Fluorescent in situ hybridization (FISH) has been widely used in the detection of tens of thousands of sperm chromosomes with its advantages of simplicity, rapidity, high sensitivity and high specificity, and has gradually become the main method of chromosome detection in sperm. Many studies using FISH technology to detect male sperm chromosomes, toxic contact history, cancer patients radiotherapy and chemotherapy, chromosome balance translocation patients and infertile men, aneuploidy rate was significantly increased compared with normal men. Especially with the application of intracytoplasmic sperm injection (ICSI) in oocytes, the role of FISH in the detection of infertile male sperm chromosomes is even more pronounced. In recent years, FISH in research sperm chromosome aneuploidy progress.