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探索一种观察细胞形态结构的新实验技术和方法，了解Ｈｅｐ２细胞和人喉鳞癌组织的形态结构特点并探讨影响该实验技术的因素，以便不断完善。方法①取培养４８ｈ后的Ｈｅｐ２细胞（培养的人喉鳞癌细胞株），直接滴片法将细胞悬液滴在ＰＭＭＡ上，以同步辐射装置产生的软Ｘ射线作光源进行ＳＸＣＭ成像研究。②选择经病理确诊的喉鳞癌患者，术中取组织石蜡包埋切片，捞在１００目电镜栅网上，进行ＳＸＣＭ成像研究。③取部分喉癌组织，环氧树脂包埋超薄切片，１００目电镜栅网捞取，ＳＸＣＭ成像研究。结果①ＳＸＣＭ技术观察Ｈｅｐ２细胞，可清楚地显现细胞膜和核膜表面有很多皱褶，凹凸不平，而光镜下无法看清这种结构特点。②ＳＸＣＭ技术在观察活细胞生理状态下的形态结构时具有比光镜独特的优点；它不需要脱水、固定、染色等一系列准备工作；在观察干组织样品时与光镜达到同样的效果。③实验在进行人喉鳞癌组织电镜超薄切片ＳＸＣＭ成像时，因切片太薄，未能成功。结论ＳＸＣＭ成像与电镜的比较研究是本系列课题下一步重点研究方向之一。影响ＳＸＣＭ技术因素的多样化，尚需进一步探索，不断完善。
To explore a new experimental techniques and methods to observe the morphological structure of cells, understand Hep 2 cells and human laryngeal squamous cell carcinoma morphological characteristics and explore the factors that affect the experimental techniques, in order to continue to improve. Methods ① Hep2 cells (cultured human laryngeal squamous carcinoma cell line) cultured for 48 h were directly dripped onto PMMA by droplet method and SXCM imaging was performed with soft X-ray generated by synchrotron radiation as a light source . ② Pathologically confirmed patients with laryngeal squamous cell carcinoma were selected, paraffin-embedded sections were taken during operation and were sighted on a 100-mesh electron microscope grid for SXCM imaging. ③ take part of laryngeal cancer tissue, epoxy resin embedded ultra-thin slices, 100 mesh electron microscopy grid fishing, SXCM imaging studies. Results ①SCMV technology observed Hep 2 cells, can clearly show the cell membrane and nuclear membrane surface has a lot of folds, uneven, and light microscopy can not see this structural features. ②SXCM technology has a unique advantage over light microscopy in observing the morphological structure of living cells; it does not require a series of preparatory work such as dehydration, fixation, staining and the like; ③ experiments in human laryngeal squamous cell carcinoma microscopy SXCM imaging, because the slice is too thin, failed to succeed. Conclusion The comparative study of SXCM imaging and electron microscopy is one of the key research directions in this series of topics. Affect the diversification of SXCM technical factors, still need further exploration, continuous improvement.