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目的设计并合成查尔酮异甘草素类化合物,并评价其抗子宫颈癌活性。方法利用Claisen-Schmidt反应原理,通过微波固相合成5种目标化合物,并进行结构表征。以Si Ha(人子宫颈鳞癌细胞)和He La(子宫颈癌细胞)细胞株作为体外模型,利用MTT法考察目标化合物对宫颈癌细胞增殖的抑制活性,利用流式细胞仪测定目标化合物对宫颈癌细胞促凋亡作用。结果与结论快速高效合成了5个查尔酮异甘草素类化合物,其结构经~1H-NMR、~(13)C-NMR谱确认。目标化合物能有效抑制宫颈癌细胞增殖,促进宫颈癌细胞凋亡。
Objective To design and synthesize chalcone isoliquiritigenin compounds and evaluate their anti-cervical cancer activity. Methods Based on the Claisen-Schmidt reaction theory, five target compounds were synthesized by microwave solid-phase reaction and characterized. Using Si Ha (human cervical squamous cell carcinoma) and He La (cervical cancer cell) cell lines as an in vitro model, the inhibitory activity of the target compound on the proliferation of cervical cancer cells was examined by MTT assay, and the target compound was determined by flow cytometry Cervical cancer cells promote apoptosis. RESULTS AND CONCLUSIONS Five compounds of chalcone isoliquiritigenin were synthesized rapidly and efficiently. Their structures were confirmed by ~ 1H-NMR and ~ (13) C-NMR spectra. The target compound can effectively inhibit the proliferation of cervical cancer cells and promote the apoptosis of cervical cancer cells.