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通过抗生素标记法获得铜绿假单胞菌ZB27的抗氯霉素突变菌株,并运用喷雾和稀释平板法检测该菌的定殖能力及对杂交竹梢枯病的防控作用。结果表明,ZB27标记菌株抗氯霉素的浓度为750μg/mL,且性状稳定、回收率高。ZB27可以快速定殖到杂交竹枝叶,并长期维持稳定的定殖量;高浓度(109、107CFU/mL)的菌液定殖量大于低浓度的菌液;在叶上的定殖量大于枝干,8天时达到顶峰,到40天时仍能保持较高的水平;病原菌的接入对生防菌在叶部的定殖影响较大,对在枝干定殖影响较小。预先接种ZB27菌悬液和其次生代谢产物对杂交竹梢枯病的防效分别为70.07%和68.05%,显著高于其它处理,说明利用菌悬液或其次生代谢物防治杂交竹梢枯病均有较好的防治效果。
The anti-chloramphenicol mutant strain of Pseudomonas aeruginosa ZB27 was obtained by antibiotic labeling method. The colonization ability of the strain and its control on the blight of hybrid shoots were detected by spray and dilution plate method. The results showed that the antibacterial activity of ZB27 was 750μg / mL against chloramphenicol and its stability was high. ZB27 could quickly colonize the hybrid bamboo shoots and maintain stable colonization for a long period of time. Colonization at high concentrations (109 and 107CFU / mL) was greater than that at low concentrations. Colonization on leaves was greater than that on shoots It reached the peak at 8 days and remained at a high level by 40 days. The access of pathogenic bacteria had a great effect on the colonization of biocontrol bacteria in the leaves, but had little effect on the colonization of the branches. Preventive inoculation of ZB27 suspension and its secondary metabolites against hybrid blight were 70.07% and 68.05%, respectively, which were significantly higher than those of other treatments, indicating that bacterial suspension or its secondary metabolites could prevent and cure hybrid shoot blight Have better control effect.