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选用120对引物在汉青一号萝卜(Raphanus sativus L.cv.Han Qing NO.1)亲本间进行PCR扩增,从中筛选出3对引物10、30、63,在双亲本间能够扩增出差异互补的条带,利用3对引物对汉青一号萝卜杂种1代群体进行纯度鉴定。鉴定结果纯度为95.1%,与同批次种子的田间鉴定结果 96.0%相近,说明应用SSR分子标记技术实施萝卜杂种1代种子的纯度鉴定是可行并可靠的。
120 pairs of primers were used for PCR amplification between Raphanus sativus L.cv.Han Qing NO.1 and three pairs of primers 10, 30 and 63 were selected from them to amplify Three pairs of primers were used to identify the purity of the first generation hybrids of Hanqing No.1 radish. The purity of the identification results was 95.1%, which was similar to 96.0% of the field test results of the same batch of seeds, indicating that the purity identification of the first generation radish hybrids by SSR molecular marker technology is feasible and reliable.