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探讨在缺氧环境下 ,移居高原的平原动物Wistar大鼠红细胞 2 ,3-二磷酸甘油酸 2 ,3-DPG浓度的变化 ;方法 :将Wistar大鼠在海拔 2 2 6 0m和 42 80m地区分别饲养 40天后 ,采用西德Bochniger-Mannheim公司生产的试剂盒 ,利用紫外分光光度法 ,对大鼠红细胞 2 ,3-DPG含量进行了测定 ;结果 :在 42 80m地区大鼠红细胞 2 ,3-DPG浓度为 (5 .83± 1 .33)nmol/L ,2 2 6 0m地区为 (4 .85± 0 .74)nmol/L ,经统计学处理P <0 .0 1。同时还对玛多返回西宁 40天后Wistar大鼠红细胞 2 ,3-DPG含量做了动态观察 ,发现随着海拔高度的下降 ,2 ,3-DPG的浓度也相应地下降 ,(4 .86± 0 .84)nmol/L ;结论 :随着海拔高度的增加 ,大鼠红细胞 2 ,3-DPG浓度的适当增加 ,是机体对缺氧的一种代偿性适应性改变。
To investigate the changes of erythrocyte 2,3-diphosphate2,3-DPG levels in erythrocytes of placental Wistar rats migrating to the plateau under hypoxic conditions; Methods: Wistar rats were divided into two groups at an altitude of 2260m and 4280m After 40 days’ feeding, the content of erythrocyte 2,3-DPG in rat erythrocytes was measured by ultraviolet spectrophotometry using the kit manufactured by Bochniger-Mannheim, West Germany. RESULTS: The erythrocyte 2,3-DPG The concentration was (5.283 ± 1.33) nmol / L, and the area of 2260m was (4.85 ± 0.74) nmol / L, and the difference was statistically significant (P <0.01). At the same time, we also observed the content of erythrocyte 2,3-DPG in Wistar rats 40 days after returning to Xining, we found that the concentration of 2,3-DPG decreased correspondingly with the decreasing of altitude (4.86 ± 0 .84) nmol / L. CONCLUSION: Appropriate increase of erythrocyte 2,3-DPG concentration with the increase of altitude is a kind of compensatory adaptive change of hypoxia.