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目的：探讨铁调素对成骨细胞铁代谢指标、骨代谢指标的影响和相互关系。方法成骨细胞（ hFOB1.19）培养3 d后，使用逆转录-聚合酶链反应（RT-PCR）检测成骨细胞（hFOB1.19）的膜转铁蛋白1（ferroportin1，Fpn1）的表达；再用不同浓度的铁调素（25 noml/L，50 noml/L，100 noml/L）干预培养环境，对照组加相同体积双蒸水，干预20 h，MTT法检测细胞增殖情况；激光共聚焦扫描显微镜（ CLSM）检测成骨细胞内铁离子荧光染色后荧光强度变化情况；Von Kossa染色检测成骨细胞矿化情况；RT-PCR检测骨钙素（BGP）、I型胶原（COL1）、骨保护素（OPG）基因表达。结果1、成骨细胞存在Fpn1表达；2、铁调素对成骨细胞增殖无显著影响（ P＞0.05）；3、铁调素干预后，成骨细胞内铁离子含量增多，且与铁调素干预存剂量依赖性关系（ P＜0.05）；4、铁调素干预后，成骨细胞矿化功能增强，且与铁调素干预存剂量依赖性关系（ P＜0.05）；5、RT-PCR检测显示不同浓度铁调素干预后，各组COL1、OPG、BGP mRNA均有表达；不同浓度组的COL1、OPG、BGP mRNA表达光密度比值不同，组间密度比值比较存在统计学意义（P＜0.05），但铁调素在400 nmol/L时抑制OPG的表达（P＜0.05）。结论成骨细胞存在铁调素作用的膜转铁蛋白-1，所以铁调素干预成骨细胞培养环境后细胞内铁离子发生特异性变化，同时细胞矿化指标显著增加、细胞COL1、OPG、BGP mRNA表达含量增加；研究提示：铁调素可能通过膜转铁蛋白影响成骨细胞铁离子变化，细胞铁代谢变化可能引起细胞成骨指标上调改变。“,”Objective To investigate the effect of hepcidin on iron and bone metabolism indexes in osteoblasts and their correlation . Methods After 3-day culturing, the expression of ferroportin1 (Fpn1) in human fetal osteoblasts hFOB1.19 was detected using reverse transcriptase-polymerase chain reaction (RT-PCR).Then, different concentrations of hepcidin (25 noml/L, 50 noml/L, and 100 noml/L) were additioned to the medium , and the same volume of double distilled water was additioned in control group .After 20 hour intervention , cell proliferation was detected using MTT method .The changes of fluorescence intensity after fluorescence staining of intracellular iron in osteoblasts were measured using a confocal laser scanning microscope ( CLSM) .von Kossa staining was performed to evaluate cell mineralization.The expression of BGP, COL1, and OPG was detected using RT-PCR.Results The expression of Fpn-1 was positive in hFOB 1.19.Hepcidin had no significant effect on hFOB 1.19 proliferation (P>0.05).After the intervention with hepcidin , the concentration of ion increased in osteoblasts , and the mineralization also enhanced , both showing a dose-dependent manner with the concentration of hepcidin ( P<0.05 ) .After the intervention with different concentrations of hepcidin , the mRNA expression of COL1, OPG, and BGP detected using RT-PCR was positive in all groups .The optical density ratio of COL1, OPG, and BGP after the intervention with different concentrations of hepcidin was different , and the difference was significant ( P<0.05 ) .The expression of OPG was inhibited by hepcidin at the concentration of 400 nmol/L (P<0.05).Conclusion Fpn1, which can react with hepcidin, exsits in osteoblasts.So, after the intervention of hepcidin , the intracellular concentration of iron ion changes specificly.And the mineralization enhances.The mRNA expression of BGP, COL1, and OPG also increase.Hepcidin may affect the changes of iron ion concentration through Fpn 1 in osteoblasts , and the changes of iron metabolism may result in the up-regulation of osteogenesis indexes .