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目的:研究人疱疹病毒6型(HHV-6)感染与淋巴瘤的关系。方法:用间接免疫荧光法及PCR,分别检测淋巴瘤患者和对照组患者血清中抗HHV-6 IgG及外周血单个核细胞(PBMC)中HHV-6 DNA序列。用免疫组化染色法,检测淋巴瘤患者淋巴结组织标本中HHV-6抗原。结果:淋巴瘤患者血清抗HHV-6抗体的阳性率为95.5％,几何平均滴度为1:123,PBMC中HHV-6 DNA的检出率为59.1％,均明显高于对照组(P<0.05)。免疫组化染色检测的5例淋巴瘤患者淋巴结组织标本中,4例HHV-6抗原阳性。结论:HHV-6感染可能与淋巴瘤的发病有关。
Objective: To study the relationship between human herpesvirus type 6 (HHV-6) infection and lymphoma. Methods: The anti-HHV-6 IgG and HHV-6 DNA in peripheral blood mononuclear cells (PBMCs) were detected by indirect immunofluorescence and PCR in patients with lymphoma and controls. Immunohistochemical staining was used to detect HHV-6 antigen in lymph node tissues of patients with lymphoma. Results: The positive rate of anti-HHV-6 antibody in lymphoma patients was 95.5%, the geometric mean titer was 1: 123, and the detection rate of HHV-6 DNA in PBMC was 59.1%, which were significantly higher than that of the control group (P < 0.05). Immunohistochemical detection of lymphoma in 5 cases of lymph node tissue specimens, 4 cases of positive HHV-6 antigen. Conclusion: HHV-6 infection may be related to the incidence of lymphoma.