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【目的】通过观察反复呼吸道感染(RRTI)非急性感染期肺脾气虚、肺脾气阴两虚型患儿(简称“复感儿”)T细胞CD8+CD+28的表达,探讨两个证型T淋巴细胞亚群的表达特点及临床意义,为RRTI的中医辨证提供可能的物质基础及客观依据。【方法】选择RRTI非急性期肺脾气虚、肺脾气阴两虚患儿各30例,运用流式细胞仪对RRTI患儿外周血T淋巴细胞亚群表达进行检测,并与正常对照组30例比较,结果运用SPSS/PC10.0统计软件包进行分析处理。【结果】与正常对照组比较,RRTI的CD8+、CD28+、CD8+CD28+%、CD8+CD28+表达降低,而CD8+CD28-%、CD8+CD28-表达增高。RRTI不同中医分型T细胞亚群的表达也不同。经多元回归分析,中医分型证候积分与CD8+CD28+%、CD8+CD28-%的表达相关。【结论】T淋巴细胞亚群失衡为RRTI发病机制之一,且与RRTI非急性感染期肺脾气虚证与肺脾气阴两虚证的证候积分相关,这可能将有助于中医辨证分型。
【Objective】 To investigate the expression of T cell CD8 + CD + 28 in patients with lung-spleen-qi deficiency and lung-Qi-Yin-Yin Deficiency syndrome during non-acute infection of recurrent respiratory tract infection (RRTI) The expression characteristics and clinical significance of syndrome T lymphocyte subsets provide a possible material basis and objective basis for RRTI TCM syndrome differentiation. 【Methods】 Thirty patients with RRTI of non-acute lung injury, spleen deficiency and deficiency of lung, spleen and qi deficiency were selected. The expression of T lymphocyte subsets in peripheral blood of children with RRTI was detected by flow cytometry and compared with the normal control group Comparison, the results using SPSS / PC10.0 statistical package for analysis and processing. 【Results】 Compared with the normal control group, the expression of CD8 +, CD28 +, CD8 + CD28 +, CD8 + CD28 + of RRTI decreased and the expression of CD8 + CD28 -% and CD8 + CD28- increased. RRTI different TCM classification of T cell subsets expression is also different. By multivariate regression analysis, TCM syndrome score was correlated with the expression of CD8 + CD28 +% and CD8 + CD28-%. 【Conclusion】 The imbalance of T lymphocyte subsets is one of the pathogenesis of RRTI, and is related to the syndrome score of pulmonary spleen-qi Deficiency Syndrome and lung-Qi-Yin Deficiency Syndrome in the period of non-acute RRTI infection, which may be helpful to TCM syndrome differentiation .