论文部分内容阅读
目的:探讨葡萄籽原花青素(GSP)对小鼠睾丸扭转复位后生精功能的保护作用。方法:24只健康雄性昆明小白鼠(8周龄,25～27 g)随机分为3组:对照组、扭转组、治疗组,每组8只。扭转组及治疗组建立单侧睾丸扭转复位动物模型,治疗组于扭转复位前30 min腹腔注射GSP(50 mg/kg),术后采用腹腔注射方式连续给药3 d,每天1次,每次50 mg/kg。扭转组方法同治疗组,治疗同体积生理盐水。术后第4天取扭转侧睾丸,检测组织病理学参数和生精细胞凋亡指数(AI),并检测睾丸组织超氧化物歧化酶(SOD)和丙二醛(MDA)含量。对照组行假手术。结果:治疗组与扭转组相比,Johnsen评分上升[(7.38±0.92)分vs(5.00±1.85)分,P<0.05],生精小管直径略增大[(178.75±1.58)μm vs(176.50±1.60)μm,P>0.05],生精细胞层数增加[(5.75±0.71)层vs(3.75±1.03)层,P<0.05],生精细胞凋亡指数AI明显降低[(16.25±1.67)%vs(40.50±1.60)%,P<0.05)],SOD活性明显上升[(52.67±3.57)U/mg prot vs(29.04±4.46)U/mg prot,P<0.05],MDA含量明显下降[(2.91±0.04)nmol/mg prot vs(4.63±0.05)nmol/mg prot,P<0.05]。结论:GSP对小鼠睾丸扭转复位后生精功能损伤有明显的保护作用,其作用机制可能与其能清除氧自由基、抑制脂质过氧化、提高机体抗氧化能力有关。
Objective: To investigate the protective effect of grape seed proanthocyanidins (GSP) on the function of spermatogenesis after testicular torsion reduction in mice. Methods: Twenty-four healthy male Kunming mice (8 weeks old, 25-27 g) were randomly divided into 3 groups: control group, torsion group and treatment group, 8 in each group. Twist group and treatment group were established unilateral testicular torsion reduction animal model, the treatment group 30 minutes before the torsion reduction intraperitoneal injection of GSP (50 mg / kg), after the administration by continuous intraperitoneal injection of 3 d, 1 time per day 50 mg / kg. Torsion group method with the treatment group, the same volume of saline treatment. Torsion testis was taken on the 4th day after operation. Histopathological parameters and apoptosis index (AI) were detected. The contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in testis were detected. Control group sham operation. Results: The Johnsen score increased by (7.38 ± 0.92) vs (5.00 ± 1.85) points, P <0.05, and the diameter of the seminiferous tubule increased slightly compared with the untreated group (178.75 ± 1.58 μm vs 176.50 P <0.05), and the apoptotic index AI of spermatogenic cells was significantly decreased ([16.25 ± 1.67] ± 1.60 μm, P> 0.05) ) (P <0.05). Compared with the control group, the activity of SOD increased obviously ([(52.67 ± 3.57) U / mg prot vs (29.04 ± 4.46) U / mg prot, P < [(2.91 ± 0.04) nmol / mg prot vs (4.63 ± 0.05) nmol / mg prot, P <0.05]. CONCLUSION: GSP has a significant protective effect on the functional impairment of spermatozoa in mice after testicular torsion and detorsion, and its mechanism may be related to its ability to scavenge oxygen free radicals, inhibit lipid peroxidation and improve the body’s antioxidant capacity.