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背景:缺血再灌注对正常组织具有损伤作用,但是它对于肿瘤组织的影响以及在肿瘤患者术后康复中的作用还不清楚。目的:探讨缺血再灌注对肿瘤组织的影响及其在肿瘤患者术后康复中的意义。设计:采用完全随机对照研究。地点和对象:实验在第四军医大学唐都医院实验外科进行,参加人员为本科全体医护人员。实验动物为第四军医大学动物实验中心提供的36只成年新西兰白兔(雌雄不拘)。干预:通过超声引导将VX2肿瘤组织混悬液穿刺注射到新西兰兔肝脏左中叶,建立肝脏肿瘤模型,用无损伤血管钳阻断肿瘤所在肝叶的肝动脉分支60min后去除血管阻断恢复血流,造成肝脏肿瘤的缺血再灌注损伤模型。主要观察指标:各组实验动物的肝脏组织和肿瘤组织的超氧化物歧化酶(SOD)和丙二醛(MDA)的含量。结果:肝脏组织中的SOD含量于缺血再灌注后迅速下降,至0min达最低点,随后有所升高,至7d时仍明显低于缺血再灌注前水平,各组与对照组对比差异显著(t=24.83～65.01,P<0.01),而肿瘤组织的SOD含量变化趋势除了1h达最低点外其余皆与肝脏组织相似,各组与对照组对比差异显著(t=4.68～12.42,P<0.01);肝脏组织的MDA含量于0min时升至最高,随后开始下降,至7d时仍高于缺血再灌注前水平,各组与对照组对比差异显著(t=7.39～42.05,P<0.01),而肿瘤组织的MDA?
BACKGROUND: Ischemia reperfusion has a damaging effect on normal tissues, but its effect on tumor tissue and its role in postoperative recovery of tumor patients remains unclear. Objective: To investigate the effect of ischemia-reperfusion on tumor tissue and its significance in postoperative rehabilitation of cancer patients. Design: Complete randomized controlled study. Location and object: The experiment was performed in Experimental Surgery of Tangdu Hospital of the Fourth Military Medical University. All the staff were undergraduate medical staff. Experimental animals for the Fourth Military Medical University Animal Experimental Center provided 36 adult New Zealand white rabbits (both male and female). Intervention: The VX2 tumor tissue suspension was punctured and injected into the left middle lobe of New Zealand rabbits by ultrasound guidance. The liver tumor model was established. The non-injured vascular clamp was used to block the hepatic artery branches of the hepatic lobe where the tumor was located. , Resulting in a model of ischemia-reperfusion injury in liver tumors. MAIN OUTCOME MEASURES: The contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in liver tissue and tumor tissue of experimental animals in each group. Results: The content of SOD in liver tissue decreased rapidly after ischemia-reperfusion, reached the lowest point at 0 min, then increased, and remained significantly lower than that before ischemia-reperfusion at 7 d. There was significant difference between each group and the control group (T = 24.83 ~ 65.01, P <0.01). However, the trend of SOD in tumor tissues was similar to that in liver except 1h, the difference was statistically significant (t = 4.68-12.42, P <0.01). The content of MDA in liver increased to the highest level at 0min and then began to decrease, which was still higher than that before ischemia-reperfusion on the 7th day. The difference between the two groups was significant (t = 7.39 ~ 42.05, P < 0.01), while the tumor tissue MDA?