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目的:探讨Stathmin/oncoprotein18(Op18)蛋白在宫颈癌的表达及意义。方法:应用免疫组化和Westernblot法检测15例正常宫颈组织、26例宫颈上皮内瘤变组织和52例宫颈癌组织中Stathmin蛋白的表达。结果:用免疫组化检测Stathmin蛋白在正常宫颈组织、宫颈上皮内瘤变及宫颈癌的阳性表达率分别为20.00%、53.85%、84.62%。正常宫颈组织组分别与宫颈上皮内瘤变及宫颈癌组比较,差异均有统计学意义(P<0.05);宫颈上皮内瘤变组与宫颈癌组比较,差异有统计学意义(P<0.05)。Westernblot法检测显示,正常宫颈组分别与宫颈上皮内瘤变组、宫颈鳞癌组及宫颈腺癌组比较,差异均有统计学意义(P<0.01),宫颈上皮内瘤变组分别与宫颈鳞癌组及宫颈腺癌组比较,差异均有统计学意义(P<0.01)。Stathmin与宫颈癌的临床分期、组织分级、区域淋巴结转移有关。结论:Stathmin在宫颈癌过表达,与宫颈癌的发生、发展密切相关。
Objective: To investigate the expression of Stathmin / oncoprotein18 (Op18) in cervical cancer and its significance. Methods: The expressions of Stathmin in 15 cases of normal cervical tissue, 26 cases of cervical intraepithelial neoplasia and 52 cases of cervical cancer were detected by immunohistochemistry and Western blot. Results: The positive rates of Stathmin protein in normal cervical tissue, cervical intraepithelial neoplasia and cervical cancer detected by immunohistochemistry were 20.00%, 53.85% and 84.62%, respectively. There were significant differences between normal cervical tissue group and cervical intraepithelial neoplasia group and cervical cancer group (P <0.05). There was significant difference between cervical intraepithelial neoplasia group and cervical cancer group (P <0.05) ). Westernblot assay showed that there were significant differences between normal cervix group and cervical intraepithelial neoplasia group, cervical squamous cell carcinoma group and cervical adenocarcinoma group (P <0.01). Cervical intraepithelial neoplasia group and cervical squamous cell carcinoma group The differences between the two groups were statistically significant (P <0.01). Stathmin and cervical cancer clinical stage, histological grade, regional lymph node metastasis. Conclusion: Stathmin overexpression in cervical cancer is closely related to the occurrence and development of cervical cancer.