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在检测人血白蛋白成品每瓶蛋白质总量时,需先称出每瓶液体总重和每毫升液体重量,求出每瓶溶液的总体积,再根据实际测得的蛋白浓度,才能求出每瓶蛋白质总量。此种方法操作繁琐。本文从人白蛋白的浓度与每毫升重量之间存在相关性着手,试图简化人白蛋白成品蛋白质总量的检测方法。样品为5％～25％的国产人白蛋白148批,人胎盘白蛋白10批,其中包括三种工艺(E法41批、AS法21批、R法96批)和两种剂型(液体124批、冻干34批)的制品。蛋白质总量测定:采用1/10000天平称出每瓶蛋白溶液的总重A和每毫升重量B,用凯氏定氮法测得蛋白浓度C,再算出每瓶蛋白质总量D[D=A/B×C/100]。结果以C为自变量X,B为因变量y(详见表1),做统计学分析,求出相关系数r=0.9999,说明C与
When testing the total amount of protein in each bottle of human albumin, it is necessary to first weigh the total weight of the liquid in each bottle and the weight of the liquid per ml to find the total volume of the solution in each bottle. Then, based on the actual measured protein concentration, Total protein per bottle. This method is cumbersome to operate. This article starts with the correlation between the concentration of human albumin and the weight per milliliter, trying to simplify the method of detecting the total protein of human albumin product. The samples were made up of 148 batches of domestic human albumin and 10 batches of human placenta albumin in 5% -25% samples, including three kinds of techniques (41 batches in E method, 21 batches in AS method and 96 batches in R method) and two dosage forms Batch, freeze-dried 34 batches) of the product. Determination of total protein: using 1/10000 balance out of each bottle of protein solution weight A and weight per milliliter B, Kjeldahl method using the protein concentration C, and then calculate the total protein per bottle D [D = A / B × C / 100]. The results of C as the independent variable X, B as the dependent variable y (see Table 1), do statistical analysis, find the correlation coefficient r = 0.9999, that C and