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目的探讨黄芪注射液对大鼠红核神经元逆行溃变的影响。方法将48只SD大鼠随机分为4组:正常组、假手术组、实验组和模型对照组,每组12只。假手术组在脊髓C3、C4之间切开黄韧带。实验组和模型对照组在脊髓C3、C4之间外侧索横断损伤红核脊髓束,随后分别以黄芪注射液和生理盐水进行腹腔注射。4周后,各组取6只大鼠将轴突示踪剂生物素葡聚糖胺(BDA)注入红核进行顺行示踪,检测轴浆顺行运输能力;另外6只大鼠行尼氏染色显示红核神经元形态及数目。结果实验组和模型对照组被BDA标记轴突的相对面积及数目低于正常组或假手术组(均P<0.01),实验组被BDA标记轴突的相对面积及数目明显高于模型对照组(均P<0.01);实验组和模型对照组存活的红核神经元数目和平均截面积均低于正常组或假手术组(均P<0.01),实验组的红核神经元数目和平均截面积均高于模型对照组(均P<0.01)。结论黄芪注射液可通过改善轴浆运输能力,提高胞体的存活率,从而对逆行溃变的红核神经元具有保护作用。
Objective To investigate the effect of Astragalus injection on the retrograde degeneration of rat red nucleus neurons. Methods 48 SD rats were randomly divided into 4 groups: normal group, sham operation group, experimental group and model control group, 12 rats in each group. The sham operation group cut the ligamentum flavum between the spinal cord C3 and C4. The experimental group and the model control group trapeze the spinal cord of the red nucleus tracts by the lateral lateral cable between the C3 and C4 of the spinal cord, and then injected intraperitoneally with astragalus injection and normal saline respectively. After 4 weeks, 6 rats in each group were injected with axon tracer biotin-dextran amine (BDA) into the red nucleus for tracing, and the ability of axoplasm to transport in the right direction was measured. In addition, 6 rats Staining showed the morphology and number of red nucleus neurons. Results The relative area and number of BDA-labeled axons in experimental group and model control group were lower than those in normal group or sham operation group (all P <0.01). The relative area and number of BDA-labeled axons in experimental group and model control group were significantly higher than those in model control group (All P <0.01). The number of red nucleus neurons and the average cross-sectional area of the experimental group and the model control group were lower than those of the normal group or the sham operation group (all P <0.01). The number of red nucleus neurons and the mean The cross-sectional area was higher than the model control group (all P <0.01). Conclusion Astragalus injection can protect the retrograde degeneration of red nucleus neurons by improving axoplasmic transport ability and increasing the survival rate of somatic cells.