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我们将各种不同浓度的全氟辛酰胺丙基三乙氧基硅烷溶液,分别与YWG(无定形硅胶,粒度5μ)反应,制备了各种不同有机层覆盖量的全氟酰胺键合YWG,其键合基为全氟辛酰胺丙基一乙氧基硅烷,有机层的表面浓度分别为0.24,0.61,0.88,1.26μmol/m~2.这些高效液体色谱填料随着所键合有机氟基团量的增加,其极性逐渐减弱,对一些甾族极性样品所给予的保留时间愈来愈短,对滞留时间愈长的样品所产生的影响愈明显.由于此种填料在硅原子上键合了氟链,增加了憎水性,可改进极性甾族化合物峰形的对称性及拖尾因子,此填料对一些在YWG柱上不能分离的胆甾烯样品有较好的分离作用,也能用于多羟基的甾族样品的分离.
We have different concentrations of perfluorooctamidopropyl triethoxysilane solution, respectively, and YWG (amorphous silica, particle size 5μ) to prepare a variety of organic layer coverage perfluorinated amide bonding YWG, The bonding group is perfluorooctamide propyl monopropoxy silane, the organic layer surface concentrations were 0.24,0.61,0.88,1.26μmol / m ~ 2. These efficient liquid chromatographic packing with the bonded organofluorine The increase of the amount of the group, its polarity gradually weakened, the retention time given to some of the steroidal polar samples given shorter and more obvious impact on samples with longer residence time due to the filler on the silicon atom The fluorine chain is bonded to increase the hydrophobicity and improve the symmetry and tailing factor of the peak shape of the polar steroid. The filler has good separation effect on some cholestane samples that can not be separated on the YWG column, It can also be used for the separation of polyhydroxy-steroidal samples.