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本实验采用成年雌性大鼠,每天取其阴道涂片检查动情周期.所有大鼠均有规则的4天周期即动情前期、动情期、动情间期Ⅰ、动情间期Ⅱ各占一日.5只动物一组,分别在动情前期第十五、二十小时,动情期第十、十五小时,动情间期Ⅰ和动情间期Ⅱ的各第十五小时于乙醚麻醉下颈动脉放血处死.在1.5分钟内取出子宫,立即放于液氮中冷冻称重后,用玻璃匀浆器将其制成组织匀浆.在辅助因素(肾上腺素2×10~(-3)M,还原型谷胱甘肽1.6×10~(-3)M)和底物花生四烯酸(2×10~(-5)M)存在下,加入制备的组织匀浆1ml,在37℃孵育20分钟.底
In this experiment, adult female rats were used to take vaginal smear test every day estrus cycle.All rats have a regular 4-day cycle of estrus, estrus, estrus Ⅰ, estrus Ⅱ every day .5 Animals were sacrificed in the carotid arteries of the fifteenth and twenty hours of estrus, tenth and fifteen hours of estrus, estrus Ⅰ and estrus Ⅱ respectively. The uterus was removed within 1.5 minutes, immediately put it in liquid nitrogen and weighed, and then homogenized with a glass homogenizer to make the tissue homogenate.After the auxiliary factors (epinephrine 2 × 10 ~ (-3) M, 1 ml of tissue homogenate was added in the presence of glutathione 1.6 × 10 -3 M and substrate arachidonic acid at 2 × 10 -5 M for 20 minutes at 37 ° C. The bottom