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目的研究p16蛋白在食管鳞状细胞癌组织的表达及其与淋巴结转移、吸烟、酗酒等因素之间的关系。方法收集河北医科大学第四医院2010年11月至2011年11月食管鳞癌患者癌组织102例作为实验组,并记录患者淋巴结转移、吸烟、酗酒等情况;内镜查体及志愿者食管正常黏膜组织96例作为对照组。通过western blot方法检测2组组织中p16蛋白的表达。结果 p16蛋白表达与年龄、性别无关(P>0.05)。实验组p16蛋白阳性表达率与对照组相比有统计学差异(44.12%vs.94.79%,P<0.05)。实验组中伴有淋巴结转移者p16蛋白阳性表达率与无淋巴结转移者相比有统计学差异(28.19%vs.71.05%,P<0.05),且p16蛋白阳性率与吸烟、酗酒有关(P<0.05)。结论 p16蛋白在食管癌组织中低表达,与吸烟、酗酒不良生活习惯有关,可作为临床诊断及判断恶性程度的辅助指标。
Objective To study the expression of p16 protein in esophageal squamous cell carcinoma and its relationship with lymph node metastasis, smoking, alcohol abuse and other factors. Methods 102 patients with esophageal squamous cell carcinoma from November 2010 to November 2011 in the Fourth Hospital of Hebei Medical University were collected as the experimental group and the patients with lymph node metastasis, smoking, alcohol abuse were recorded. The endoscopic examination and normal esophageal of the volunteers 96 cases of mucosal tissue as a control group. The expression of p16 protein in two groups of tissues was detected by western blot. Results The expression of p16 protein was not related to age and gender (P> 0.05). The positive rate of p16 protein in experimental group was significantly higher than that in control group (44.12% vs94.79%, P <0.05). The positive rate of p16 protein in the experimental group was significantly higher than that in the non-lymph node metastasis (28.19% vs.71.05%, P <0.05), and the positive rate of p16 protein was related to smoking and alcohol abuse (P < 0.05). Conclusion The low expression of p16 protein in esophageal cancer tissue is related to the bad habits of smoking and alcohol abuse, which can be used as an auxiliary index for clinical diagnosis and malignancy.