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目的 :探讨卡介苗 (BCG)对卵蛋白 (OVA)致敏小鼠肺组织中T细胞的在体调节及其对气道炎症的作用。方法 :将 30只BALB/c小鼠分为 3组 ,第 1组吸入雾化的OVA (1次 /d ,2 0min/次 ,连续 10d) ,建立致敏模型。第 2组 (对照 )吸入雾化的生理盐水 (时间和次数同第 1组 ) ;第 3组 (治疗组 )于致敏前 10d及 14d ,各皮内注射BCG 1次 ,致敏后 6d吸入雾化的纯蛋白衍生物 (PPD)。用SABC免疫组化法 ,检测肺组织中CD4 + 、CD8+ 及IFN γ+ 细胞的变化 ,以及肺组织和支气管肺泡灌洗液 (BALF)中炎性细胞的变化。结果 :OVA致敏组小鼠肺组织中CD4 + T细胞增加 ,CD8+ T细胞无明显变化 ,主要表现为IFN γ-/CD4 + T细胞数的增加 ,IFN γ+ /CD4 + 细胞的比例降低。BCG治疗后 ,肺组织中CD4 + T细胞减少 ,CD8+ T细胞数大量增加 ;IFN γ+ /CD4 + 细胞的比例明显增大 ;BALF中炎性细胞数减少。结论 :BCG可在体上调Th1细胞 ,减轻实验性哮喘动物气道的炎症反应
Objective: To investigate the in vivo regulation of BCG on T cells in the lung tissue of OVA-sensitized mice and its effect on airway inflammation. Methods: Thirty BALB / c mice were divided into three groups, the first group inhaled nebulized OVA (1 / d, 20min / time, continuous 10d), the establishment of sensitization model. Group 2 (control) inhaled nebulized saline (group 1); Group 3 (treatment group) injected BCG once intradermally 10 d and 14 d before sensitization, and inhaled 6 d after sensitization Atomized neat protein derivative (PPD). The changes of CD4 +, CD8 + and IFNγ + cells in lung tissue and the changes of inflammatory cells in lung tissue and bronchoalveolar lavage fluid (BALF) were detected by SABC immunohistochemistry. Results: The number of CD4 + T cells in lung tissue of OVA sensitized mice increased but there was no significant change in CD8 + T cells. The main manifestations were IFN γ- / CD4 + T cell number and IFN γ + / CD4 + cell ratio decreased. After the treatment of BCG, the number of CD4 + T cells and the number of CD8 + T cells in lung tissue increased significantly; the proportion of IFNγ + / CD4 + cells increased significantly; and the number of inflammatory cells in BALF decreased. CONCLUSION: BCG can up-regulate Th1 cells in vivo and reduce the airway inflammatory response in experimental asthmatic animals