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目的建立测定定喘止嗽丸中可待因含量的HPLC法。方法采用HPLC法,以Kromasil C8(5μm,250mm×4.6mm)为色谱柱;流动相:乙腈-0.01mol·L~(-1)磷酸氢二钾溶液-0.005mol·L~(-1)庚烷磺酸钠水溶液(24∶38∶38);检测波长:220nm(带宽4nm);流速:1.0ml·min~(-1)。结果可待因的加样回收率平均为97.54%,RSD为0.91%(n=6)。结论试验结果表明,本方法准确、灵敏度高、重复性好,可用于定喘止嗽丸的质量控制。
Objective To establish an HPLC method for the determination of codeine in Dingchuan Zhisuo pill. Methods The HPLC method was used with Kromasil C8 (5μm, 250mm × 4.6mm) as the mobile phase. The mobile phase was acetonitrile-0.01mol·L -1 dipotassium hydrogen phosphate solution-0.005mol·L -1 G Sodium alkanesulfonate solution (24:38:38); detection wavelength: 220nm (bandwidth 4nm); flow rate: 1.0ml · min -1. Results The average recovery of codeine was 97.54% with RSD of 0.91% (n = 6). Conclusion The experimental results show that the method is accurate, sensitive and reproducible. It can be used for quality control of Dingchuanzhisowan pill.