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目的:检测蛋白增殖细胞核抗原(PCNA)、p63和p53在肺癌组织中的表达情况,以探讨三者在肺癌的发生、发展中的生物学作用和临床意义。方法:选取195例肺癌组织(其中57例有癌旁组织),应用组织芯片技术和免疫组织化学方法观察三种蛋白的表达情况,并研究三者之间及其与临床病理参数的关系。结果:PCNA、p63和p53蛋白在肺癌组织中的阳性表达率分别为96.41%、38.46%及58.46%,但三者在癌旁组织中均无表达,差异有统计学意义(均P<0.05);在肺癌组织中,PCNA、p63和p53蛋白的表达情况均与组织分型有关(P<0.05),且PCNA、p53蛋白表达与分化程度有关(P<0.05),分化越差,表达越高;p53表达与PCNA表达呈正相关(r=0.352,P=0.043),p63与p53、PCNA的表达不相关(P>0.05)。结论:肺癌组织中PCNA、p63和p53蛋白的表达升高,三者均在肺癌的发生、发展中发挥着重要作用,并且临床可通过检测三者的蛋白水平,作为鉴别肺鳞状细胞癌与其他类型癌的重要参考指标,为病理诊断提供依据。
OBJECTIVE: To detect the expression of proliferating cell nuclear antigen (PCNA), p63 and p53 in lung cancer and to explore the biological roles and clinical significance of the three in the development and progression of lung cancer. Methods: 195 cases of lung cancer tissues (57 cases of adjacent tissues) were selected. The expression of three proteins was observed by tissue microarray and immunohistochemistry. The relationship between the three proteins and clinicopathological parameters was also studied. Results: The positive rates of PCNA, p63 and p53 in lung cancer tissues were 96.41%, 38.46% and 58.46%, respectively, but none of them was detected in adjacent tissues (P <0.05) . The expression of PCNA, p63 and p53 in lung cancer was correlated with the histological type (P <0.05), and the expression of PCNA and p53 was related to the degree of differentiation (P <0.05). The worse the differentiation and the higher the expression ; The expression of p53 was positively correlated with the expression of PCNA (r = 0.352, P = 0.043), while the expression of p63 was not correlated with the expression of p53 and PCNA (P> 0.05). CONCLUSIONS: The expression of PCNA, p63 and p53 in lung cancer tissues is elevated, all of which play an important role in the occurrence and development of lung cancer. The levels of PCNA, p63 and p53 in lung cancer can be detected by detecting the protein levels of the three as a marker of lung squamous cell carcinoma Other types of cancer, an important reference index for the diagnosis of pathological basis.