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目的对一起食物中毒的样品进行微生物检测,查找引起食物中毒的病原菌,预防类似食源性疾病的再次发生。方法根据流行病学调查及临床表现,依据GB 4789.4—2010、GB 4789.5—2012、GB 4789.7—2013食品安全国家标准食品微生物学检验、GB/T 4789.6—2003《食品卫生微生物学检验致泻大肠埃希氏菌检验》、GB/T 4789.14—2003《食品卫生微生物学检验蜡样芽胞杆菌检验》、WS/T9—1996《变形杆菌食物中毒诊断标准及处理原则》,对采集的样品进行病原菌分离与鉴定。结果在4份卤菜食品、4份食品用具、1份销售人员手表面涂抹拭子、1份销售人员肛拭子、28份患者肛拭子中检出奇异变形杆菌,4份卤菜食品中奇异变形杆菌计数均>106cfu/g。12份患者血清分别与分离的奇异变形杆菌抗原做血清凝集试验,恢复期血清凝集效价高于急性期4倍。结论根据流行病学调查及实验室鉴定分析,本次食物中毒是由奇异变形杆菌污染卤菜食品导致。
Objective To detect the microorganism in a sample of food poisoning, find the pathogen causing food poisoning and prevent the recurrence of similar foodborne diseases. Methods According to epidemiological investigation and clinical manifestations, according to GB 4789.4-2010, GB 4789.5-2012, GB 4789.7-2013 National Food Safety Standard Microbiological Examination of Food, GB / T 4789.6-2003 “Microbiological Examination of Food Hygiene due to diarrhea and large intestine Corynebacterium test ”, GB / T 4789.14-2003“ Microbiological examination of food hygiene Bacillus cereus test ”, WS / T9-1996“ Proteus food poisoning diagnostic criteria and principles ”, the collected samples were isolated and pathogenic bacteria Identification. Results Four samples of Braised vegetable, four food utensils, one copy of the salesman’s surface swab, one copy of the sales staff anal swab and 28 samples of the patient’s anal swab were detected. Bacillus counts were> 106cfu / g. Serum of 12 patients were separately tested for the serum agglutination with the isolated Proteus mirabilis antigen, and the recovery serum agglutination titer was 4 times higher than that of the acute phase. Conclusion According to epidemiological investigation and laboratory analysis, this food poisoning is caused by the contamination of Radish by Proteus mirabilis.