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目的 探讨前脑缺血再灌注引起的海马ＣＡ_１区神经元死亡是否包含凋亡机制的参与以及缺血预处理（ＩＰＣ）是否通过影响凋亡抑抑制基因ｂｃｌ－２的表达起到脑缺血损伤保护作用。方法（１）动物模型建立及分组；选用健康成年雄性Ｗｉｓ－ｔａｒ大鼠，参照Ｐｕｌｓｉｎｅｌｌｉ的四血管阻断法建立前脑缺血模型。动物分为３组；单纯缺血再灌注组（ＩＲ组），给子假手术３ｄ后行８ｍｉｎ致死性缺血；缺血预处理组（ＩＰＣ组），先给予３ｍｉｎ短暂缺血，３ｄ后再给予８ｍｉｎ缺血；对照组，只给予假手术，不给于缺血刺激。（２）结合ＰＩ染色的流氏细胞术检测：于缺血再灌注２４ｈ。迅速取两背侧新鲜海邓组织，采用流氏细胞仪（ＦＣＭ）进行海马ＣＡ_１区神经元细胞凋亡的检测，低于Ｇ_１期ＤＮＡ含量的细胞为凋亡细胞。（３）免疫组化检测：于缺皿再灌注２ｈ，取脑制备冰冻切片，采用ＳＡＢＣ法检测各组海马ＣＡ_１区神经元ｂｃｌ－２及 ｂｃｌ－ｘｌ训蛋白的表达。结果流氏细胞术检测显示，ＩＲ组及 ＩＰＣ组缺血再灌注 ２４ｈ时海马ＣＡ_１区神经元的凋亡百分率分别为１３．１１±３．００％和２．４０±０．９８％，与对照组（凋亡百分率为Ｉ．３４±０．６２％）相比，均有显著性差异（Ｐ＜０．００?
Objective To investigate whether neuronal death in the hippocampal CA1 region caused by forebrain ischemia-reperfusion involves the mechanism of apoptosis and whether ischemic preconditioning (IPC) plays a role in cerebral ischemia injury by affecting the expression of apoptosis-suppressing gene bcl-2 Protective effects. Methods (1) Animal models were established and divided into groups. Healthy adult male Wis-tar rats were used to establish forebrain ischemia model with reference to Pulsinelli’s four-vessel occlusion method. The animals were divided into 3 groups. The ischemia-reperfusion group (IR group) was given 8-month lethal ischemia after sub-sham operation. The ischemic preconditioning group (IPC group) Given 8min ischemia; control group, only given sham surgery, do not give ischemic stimulation. (2) Flow cytometry combined with PI staining: 24h after ischemia-reperfusion. Quickly take fresh dorsal seawater tissue from both sides and detect the apoptosis of hippocampal CA1 neurons by flow cytometry (FCM). The apoptotic cells are lower than the DNA of G_1 phase. (3) Immunohistochemistry: Frozen sections were prepared at 2h after reperfusion, and the expressions of bcl-2 and bcl-xlcr in hippocampal CA1 were detected by SABC method. Results Flow cytometry showed that the apoptotic percentage of hippocampal CA1 neurons was 13.11 ± 3.00% and 2.40 ± 0.98% respectively in IR group and IPC group at 24h, compared with control Group (the percentage of apoptosis was I.34 ± 0.62%), there was a significant difference (P <0.00?