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本文应用微量直接法检测血清中 HBV DNAP并对海洋药物“911”抗 HBV作用进行了评价 ,结果表明 :PFA对DNAP有较强的抑制作用 ,5 0μg/ ml剂量能全部阻断 DNAP的活性 ,1.2 5μg/ ml能抑制 5 .6 %的 DNAP活性 ,与有关报道一致。“911”对 HBV DNAP有一定的抑制作用 ,经两次平行样本重复实验 ,其结果基本一致。其平均半数抑制剂量为 4.15 mg/ ml。证明微量直接法体外测定药物对 HBV DNAP的抑制作用 ,是评价抗 HBV药物的一种快速简便的方法。
In this paper, serum HBV DNAP was detected by micro direct method and the anti-HBV effect of “911” of marine drug was evaluated. The results showed that: PFA had a strong inhibitory effect on DNAP, 50μg / ml dose could completely block the activity of DNAP, 1.2 5μg / ml can inhibit the DNAP activity of 5.6%, consistent with the reported. “911” has a certain degree of inhibition of HBV DNAP. After repeated experiments on two parallel samples, the results are basically the same. The average half-dose inhibition was 4.15 mg / ml. Proved micro-direct method of in vitro determination of drugs on HBV DNAP inhibition, evaluation of anti-HBV drugs is a quick and easy way.