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【目的】评价束缚(immobilization,IMO)应激方法建立大鼠应激性心肌病(stress cardiomyopathy,SC)模型的有效性。【方法】健康雄性成年SD大鼠背部IMO 2 h,对照组麻醉后不束缚,检测二组大鼠心电图、左心室血流动力学、形态、血清去甲肾上腺素(norepinephrine,NE)及心肌损伤标志物(myocardial injury biomarker,cTnI)变化。【结果】大鼠IMO后5 min,与对照组比较:(1)心电图显示心率增加、ST段抬高、QTc间期延长。(2)血浆NE、cTnI浓度明显升高。(3)左心室内压、dp/dt明显升高。(4)超声心动图显示收缩期左心室心尖近球形扩张。【结论】大鼠IMO后迅速出现心电图、左心室血流动力学、左心室心尖扩张及血浆NE、cTnI浓度变化,符合SC主要临床特征。IMO应激模型建立方法简单,为SC基础研究提供了良好的动物模型。
【Objective】 To evaluate the effectiveness of immobilization (IMO) stress in establishing a stress cardiomyopathy (SC) model in rats. 【Methods】 The healthy adult male Sprague-Dawley rats were exposed to IMO for 2 h on the back of the rats and the control rats were anesthetized. The electrocardiogram, left ventricular hemodynamics, morphology, serum norepinephrine (NE) and myocardial injury (Myocardial injury biomarker, cTnI) changes. 【Results】 At 5 min after IMO, rats were compared with the control group. (1) The electrocardiogram showed that heart rate increased, ST segment elevation and QTc interval prolonged. (2) plasma NE, cTnI concentration was significantly increased. (3) Left ventricular pressure, dp / dt increased significantly. (4) echocardiography showed systolic left ventricular apical nearly spherical expansion. 【Conclusion】 Electrocardiogram, left ventricular hemodynamics, apical dilatation of left ventricle and plasma concentrations of NE and cTnI were rapidly observed in rats, which accorded with the main clinical features of SC. IMO stress model to establish a simple method for basic research SC provides a good animal model.