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用免疫组织化学法,在冰冻切片上观察了不同胎龄组肝内前B细胞的形态、分布及其分化为B细胞过程中抗原的变化。在早期胎肝(9～20周)内有较多的前B细胞,大小不一,形态各异,但有共同的抗原,如IgM、BA-1、HLA-DR和TdT均为阳性。这些细胞多数分布在胎儿肝的窦周隙,少部分分布于窦内或血管周围。第13周后开始出现IgD和IgA阳性细胞,OKB-2和Leu 14阳性细胞随之大量增加,HLA-DR,Kappa和Lambda阳性细胞也相应增加。上述阳性细胞的出现提示B细胞进一步成熟。山于胎肝缺乏抗原刺激,B细胞不能发育为浆细胞。此外,B细胞在肝内的分化、成熟与T细胞关系不大,因为在缺少T细胞的情况下,B细胞仍在发育。说明B细胞在肝内的分化、发育主要依赖肝的微环境。本研究为选用第9～20周胎肝治疗再生障碍性贫血和B细胞缺乏症提供了依据。
Immunohistochemistry was used to observe the morphology, distribution of the pre-B cells in different gestational age groups and the change of antigens in their differentiation into B cells on frozen sections. In early fetal liver (9 to 20 weeks), there are more pre-B cells of different sizes and morphologies but share common antigens such as IgM, BA-1, HLA-DR and TdT. Most of these cells are located in the fetal liver sinus perivitexual, a small part of the distribution in the sinus or perivascular. IgD and IgA positive cells began to appear after the 13th week. The numbers of OKB-2 and Leu 14 positive cells increased significantly, and the numbers of HLA-DR, Kappa and Lambda positive cells increased accordingly. The appearance of these positive cells suggests that B cells further mature. Lack of mountain antigen stimulation in fetal liver, B cells can not develop into plasma cells. In addition, B cells in the liver differentiation, maturation and T cells have little relationship, because in the absence of T cells, B cells are still developing. Explain B cells in the liver differentiation, development depends mainly on the liver microenvironment. This study for the selection of 9 to 20 weeks of fetal liver treatment of aplastic anemia and B cell deficiency provides the basis.