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哺乳动物的腺垂体是一种至少有五种不同分泌细胞类型的非均一性细胞复合体。由于技术上的困难,下丘脑促性腺激素释放激素(GnRH)对垂体促性腺细胞的电生理活动的影响,一直未能阐明。作者用羊腺垂体结节部(PT)离体制备,作为促性腺细胞的自然富集源,并证明GnRH 诱导细胞Ca~(2+)通道的活化,Ca~(2+)内流为启动这种细胞分泌激素所必需。实验用胰蛋白酶分散了的羊PT 细胞作原代体外单层培养,4～7天后,用免疫细胞化学定位技术观察
Mammalian adenohypophysis is a heterogeneous cellular complex of at least five different secreted cell types. Due to technical difficulties, the effect of hypothalamic gonadotropin-releasing hormone (GnRH) on the electrophysiological activity of pituitary gonadotrophic cells has not been elucidated. The authors used the goat pituitary nodules (PT) isolated in vitro as a natural source of gonadotrophs and GnRH-induced activation of Ca ~ (2+) channels, Ca ~ (2 +) influx was initiated This cell is necessary for the secretion of hormones. Experimental trypsin dispersed sheep PT cells as the primary monolayer culture in vitro, 4 to 7 days later, the use of immunocytochemical localization of observation