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以蛹虫草菌(Cordyceps militaris)为试材,用单因素试验筛选适宜温度后,利用响应面法优化培养基,最后用优化的条件和培养基进行摇床6d和摇床6d+静置培养10d的液体发酵试验,研究不同培养条件下蛹虫草菌液体发酵后菌丝体产率以及静置培养对虫草素积累量的影响。结果表明:蛹虫草菌适宜温度为25℃,优化培养基为蔗糖4.34%、酵母粉3.06%、硫酸亚铁0.027%、磷酸二氢钾0.2%、硫酸铵0.04%、硫酸镁0.13%、维生素B10.08%、硫酸锌0.06%;摇床6d和摇床6d+静置10d后,发酵液中的菌丝体产率分别为2.568g/100mL和3.389g/100mL,后者菌丝体产率比初始培养基增加了1.73倍,而虫草素积累量分别达到568.329μg/mL和862.893μg/mL,后者比初始培养基中增加了1.56倍。
After Cordyceps militaris was selected as the test material, the optimal temperature was selected by single factor test, and the medium was optimized by response surface methodology. The optimal conditions and medium were shaken 6d and shaker 6d + Liquid fermentation test to study mycelial yield after liquid culture of Cordyceps militaris under different culture conditions and the effects of static culture on the accumulation of cordycepin. The results showed that the suitable temperature of Cordyceps militaris was 25 ℃, the optimized medium was sucrose 4.34%, yeast powder 3.06%, ferrous sulfate 0.027%, potassium dihydrogen phosphate 0.2%, ammonium sulfate 0.04%, magnesium sulfate 0.13%, vitamin B10 .08%, and zinc sulfate 0.06%. The mycelial yield in the fermentation broth was 2.568g / 100mL and 3.389g / 100mL respectively after shaking for 6d and shaker for 6d + for 10d. The mycelial yield ratio The initial medium increased by 1.73 times, while the accumulation of cordycepin reached 568.329μg / mL and 862.893μg / mL respectively, which was 1.56 times higher than the initial medium.