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本研究旨在探讨人类非典型HLA抗原E(HLA-E)对同胞全相合造血干细胞移植(HSCT)后巨细胞病毒(CMV)感染的影响。以2005年1月至2011年1月在我院行HLA同胞全相合HSCT患者119例为研究对象,采用PCR和测序方法检测供、受体HLA-E基因多态性。结果表明,HLA-E*0101/0101纯合型占20.17%,HLA-E*0103/0103纯合型占27.73%,HLA-E*0101/0103杂合型占52.10%;HLA-E*0101/0101纯合子组CMV感染15例,CMV感染率为62.50%;HLA-E*0103/0103纯合子组CMV感染16例,CMV感染率为48.48%;HLA-E*0101/0103杂合子组CMV感染20例,CMV感染率为32.25%。含HLA-E*0103基因组与HLA-E*0101/0101纯合子组患者CMV感染率比较,差异有统计学意义(P=0.0295),与CMV疾病的发生率比较,差异仍有统计学意义(P=0.0074)。结论:HLA-E基因多态性对同胞全相合移植后CMV感染和疾病的发生存在影响。
This study aimed to investigate the effect of human atypical HLA antigen E (HLA-E) on cytomegalovirus (CMV) infection after sibling full-matched hematopoietic stem cell transplantation (HSCT). A total of 119 HLA-matched patients with HSCT in our hospital from January 2005 to January 2011 were selected as the research objects. The polymorphisms of HLA-E gene in donor and recipient were detected by PCR and sequencing. The results showed that HLA-E * 0101/0101 homozygote accounted for 20.17%, HLA-E * 0103/0103 homozygous accounted for 27.73%, and HLA-E * 0101/0103 heterozygous accounted for 52.10% In the group of HLA-E * 0103/0103 homozygote, CMV infection was found in 16 cases and the CMV infection rate was 48.48%. In the HLA-E * 0101/0103 heterozygote group, the CMV infection rate was 62.50% Infection in 20 cases, CMV infection rate was 32.25%. The difference of CMV infection rates between HLA-E * 0103 and HLA-E * 0101/0101 homozygous groups was statistically significant (P = 0.0295). The difference was still significant compared with the incidence of CMV disease P = 0.0074). Conclusion: HLA-E gene polymorphism affects the occurrence of CMV infection and disease after suturing allografts.