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目的:观察定期检测胰岛素对糖尿病大鼠表征的影响。方法:链脲佐菌素(STZ)腹腔注射50mg/kg建立糖尿病模型,观察4周。模型1组(M1组)与正常1组(N1组)每周尾静脉采血0.5mL测试胰岛素;模型2组(M2组)与正常2组(N2组)在处死后采血测试胰岛素。定期观察大鼠一般状况,检测大鼠体重、血糖、饮水量、尿量、体温、旷场活动、抓力。结果:分别与N1、N2组比较,M1、M2组大鼠体重及抓力下降、血糖升高、饮水量及尿量增加,差异有统计学意义(P<0.05);M2组大鼠体温下降,差异有统计学意义(P<0.05),毛、尾巴、爪光泽尚可;M1组大鼠体温改变不明显,毛枯槁、尾巴严重结痂、爪脱屑。结论:M1、M2组糖尿病大鼠具有不同的表征,可能与M1组定期尾静脉采血有关,提示指标检测过程会影响动物的表征。
Objective: To observe the effect of regular insulin test on the characterization of diabetic rats. Methods: Streptozotocin (STZ) was intraperitoneally injected with 50mg / kg to establish a diabetic model for 4 weeks. 0.5 mL of test insulin was collected from the tail vein of the model group 1 (M1 group) and the normal group 1 (N1 group), while the model group 2 (M2 group) and the normal group 2 (N2 group) were tested for insulin after sacrifice. Regular observation of the general condition of rats, body weight, blood glucose, water intake, urine output, body temperature, open field activity, grasping force. Results: Compared with N1 and N2 groups, the body weight and grasping power of M1 and M2 rats decreased, the blood glucose increased, the amount of drinking water and urine volume increased, the difference was statistically significant (P <0.05); the body temperature of M2 rats decreased , The difference was statistically significant (P <0.05), hair, tail, claw gloss was acceptable; M1 group rats did not change the body temperature, hair dry scabies, severe scab tail, claw scaling. Conclusion: The rats with M1 and M2 diabetes mellitus have different characteristics, which may be related to the regular tail vein blood sampling in group M1, suggesting that the detection of indicators may affect the characterization of animals.