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目的：探讨饲料锌对腹腔巨噬细胞（Ｐｅｒｉｔｏｎｅａｌｍａｃｒｏｐｈａｇｅ，ＰＭΦ）吞噬功能的影响及其机理。方法：建立大鼠缺锌、高锌模型，测定大鼠ＰＭΦ吞噬率、吞噬指数及ｃＡＭＰ、ｃＧＭＰ。结果：①缺锌（ＺＤ）组ＰＭΦ吞噬率、吞噬指数均非常显著地低于配对（ＰＦ）组及自由喂养（ＡＬ）组，经补锌后得以恢复；高锌（ＺＥ）组明显低于ＡＬ组。②ＰＭΦ经体外脂多糖（ＬＰＳ）作用后胞内ｃＡＭＰ含量、ｃＡＭＰ／ｃＧＭＰ水平ＺＤ组明显低于ＰＦ、ＡＬ及缺锌补锌（ＺＤ＋ＡＬ）组；ＺＥ组非常显著地高于ＡＬ组。结论：不适量锌可能通过ＰＭΦ胞内ｃＡＭＰ／ｃＧＭＰ变化而影响ＰＭΦ活化，进而抑制其吞噬杀菌功能。
Objective: To investigate the effects of dietary zinc on the phagocytic function of peritoneal macrophage (PMΦ) and its mechanism. Methods: Zinc-deficient and high-zinc model rats were established. The phagocytic rate, phagocytic index and cAMP, cGMP of rats were measured. RESULTS: ① The phagocytic rate and phagocytic index of PMΦ in ZD group were significantly lower than those in PF group and AL group, and were restored after Zinc supplementation. Zinc group was significantly lower than that in ZE group AL group. (2) The intracellular cAMP level and cAMP / cGMP level in PMΦ treated by lipopolysaccharide (LPS) in vitro were significantly lower than that in PF, AL and ZD + AL groups; ZE group was significantly higher than that in AL group. Conclusion: Zinc deficiency may affect the activation of PMΦ by PMΦ intracellular cAMP / cGMP changes, thereby inhibiting its phagocytosis.