论文部分内容阅读
[目的]探讨环氧合酶-2(COX-2)抑制剂塞来昔布对体外培养的肝癌细胞的抗增殖及诱导凋亡作用。[方法]培养肝癌细胞株hepG2,用不同浓度的塞来昔布进行干预,MTT法检测对细胞生长的抑制作用;流式细胞术检测细胞凋亡情况及对细胞周期的影响。[结果]与对照组相比,随着塞来昔布剂量和作用时间的增加,MTT显示细胞的吸光度值逐渐降低,塞来昔布作用24、36、48h,对hepG2细胞的中效抑制浓度(IC50)分别为94.2μmol/L、78.3μmol/L和55.7μmol/L,相关系数分别为0.97、0.94和0.99;随着塞来昔布浓度的增加和作用时间的延长,细胞的凋亡率升高;塞来昔布作用48h后,随着药物浓度的增加G0/G1期细胞比例增加,S期细胞比例下降。[结论]塞来昔布能抑制hepG2细胞增殖,促进细胞凋亡,并呈剂量和时间依赖性。
[Objective] To investigate the antiproliferative and apoptosis-inducing effects of celecoxib, a cyclooxygenase-2 (COX-2) inhibitor, on hepatoma cells cultured in vitro. [Methods] The hepatocarcinoma cell line hepG2 was cultured and treated with different concentrations of celecoxib. MTT assay was used to detect the inhibitory effect on cell growth. Flow cytometry was used to detect the cell apoptosis and cell cycle. [Results] Compared with the control group, MTT showed that the cell absorbance decreased gradually with the dose and time of celecoxib, and the effect of celecoxib at 24, 36 and 48 hours on the inhibitory concentration of hepG2 cells (IC50) were 94.2μmol / L, 78.3μmol / L and 55.7μmol / L respectively, the correlation coefficients were 0.97, 0.94 and 0.99 respectively. With the increase of celecoxib concentration and the prolongation of action time, After 48 hours of celecoxib treatment, with the increase of drug concentration, the proportion of cells in G0 / G1 phase increased and the proportion of S phase cells decreased. [Conclusion] Celecoxib can inhibit hepG2 cell proliferation and promote apoptosis in a dose and time dependent manner.