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目的探讨白藜芦醇对高糖高脂饮食大鼠肾氧化损伤的保护作用。方法大鼠分为正常对照组、高糖高脂组、白藜芦醇低、中、高剂量组,13周后处死大鼠,测定血糖血脂、肾组织氧化应激水平、抗氧化酶活性及肾脏过氧化物酶激活受体α(PPARα)、羟甲基戊二酸单酰辅酶A合成酶(HMGCS2)mRNA表达水平。结果高糖高脂饮食13周后,高糖高脂组大鼠体重为(453.00±19.54)g,明显高于正常对照组(368.75±25.24)g和高剂量白藜芦醇组(400.40±30.39)g(t=4.545、2.991,P<0.05);高糖高脂组大鼠肾脏总抗氧化能力(T-AOC)、超氧化物歧化酶(T-SOD)分别为(1.22±0.34)、(2.69±0.13)U/(mg.Pro),明显低于正常对照组的(0.27±0.03)、(2.43±0.07)U/(mg.pro)(t=3.491,P=0.003;t=2.793,P=0.011)。结论高糖高脂饮食大鼠肾内处于明显的氧化应激状态,白藜芦醇对高糖高脂饮食引起的氧化性肾损伤有保护作用。
Objective To investigate the protective effect of resveratrol on renal oxidative damage in rats with high glucose and high fat diet. Methods Rats were divided into normal control group, high glucose and high fat group, resveratrol low, medium and high dose groups, rats were sacrificed after 13 weeks, blood glucose, lipid peroxidation, oxidative stress in renal tissue, antioxidant enzyme activity and Renal peroxidase-activated receptor α (PPARα), HMGCS2 mRNA expression levels. Results After 13 weeks of high glucose and high fat diet, the body weight of rats in high glucose and high fat group was (453.00 ± 19.54) g, which was significantly higher than that in normal control group (368.75 ± 25.24g) and high dose resveratrol group (400.40 ± 30.39) ) (t = 4.545, 2.991, P <0.05). The total antioxidant capacity (T-AOC) and superoxide dismutase (T-SOD) in high glucose and high fat group were (1.22 ± 0.34) (2.69 ± 0.13) U / (mg.Pro), which was significantly lower than that of the control group (0.27 ± 0.03), (2.43 ± 0.07) U / (mg.pro) (t = 3.491, P = 0.003; , P = 0.011). Conclusion High glucose and high fat diets in rat kidney are obviously oxidative stress, and resveratrol has a protective effect on oxidative renal injury induced by high glucose and high fat diet.