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目的对喂饲经辐照和高压两种不同灭菌处理方法饲料大鼠的子代的生理生长、神经反射指标和学习记忆功能进行比较研究,为实验动物饲料灭菌方法的取舍、限制提供参考依据。方法试验设2个组,分别为喂饲高压蒸汽灭菌饲料组和喂饲辐照灭菌饲料组。给亲代大鼠分别喂饲高压蒸汽灭菌饲料和辐照灭菌饲料10周后,合笼交配,自然分娩产仔。每组选15窝,每窝选8只仔鼠,测试仔代大鼠生理生长指标(张耳、开眼、长毛、出牙时间)和神经反射指标(平面翻正、前肢抓力、悬崖回避、听觉警戒、负趋地性、视觉发育、空中翻正);于6周龄时,各组选择雄性大鼠15只分别进行避暗试验和穿梭试验,测试学习记忆功能指标(避暗潜伏期、错误次数、穿梭主动回避次数、主动回避时间)。实验数据以SPSS软件统计分析。结果避暗试验中,摄食辐照饲料大鼠在记忆巩固和记忆消退试验中,潜伏期(150.0±62.7)s和(126.6±61.8)s均长于摄食高压饲料大鼠(65.9±83.9)s和(56.0±53.7)s(P<0.05),错误次数(0.2±0.4)次和(0.5±0.6)次均小于摄食高压饲料大鼠(0.9±0.9)次和(1.4±0.9)次(P<0.05),其余各项生理生长、神经反射指标和学习记忆功能指标差异均无统计学意义(P>0.05)。结论喂饲辐照饲料和高压饲料的大鼠子代生长发育及神经反射方面差异无统计学意义,被动记忆方面喂饲辐照饲料子代大鼠表现优于喂饲高压饲料。
OBJECTIVE To compare the physiological growth, reflex indices and learning and memory function of offspring fed with radiation and high pressure two different methods of sterilization, to provide a reference for the selection and restriction of experimental animal feed sterilization methods in accordance with. Methods Two groups were set up, which were fed with high-pressure steam sterilization group and with irradiated irradiation-sterilized feed group. After feeding the autophagy rats with autoclaved feed and irradiated sterilized feed for 10 weeks, the cages were mated and naturally delivered. Each group of 15 litters, each litter selection of 8 offspring, offspring rats physiological growth indicators (Zhang Er, open eyes, hairy, teething time) and neurological reflex indicators (plane correction, forelimb grip, cliff avoidance At 6 weeks of age, 15 male rats in each group were randomly divided into darkness-avoidance test and shuttle test to test the learning and memory function (latency avoidance latency, The number of mistakes, shuttle active avoidance times, active avoidance time). Experimental data to SPSS software statistical analysis. Results During the dark-avoidance test, the incubation periods (150.0 ± 62.7) s and (126.6 ± 61.8) s were longer in rats fed irradiated diet than those fed high-fed diet (65.9 ± 83.9) s and 56.0 ± 53.7 s (P <0.05), the number of errors (0.2 ± 0.4) and (0.5 ± 0.6) times were significantly lower than those of 0.9% 0.9 and 0.9 ± 0.9 (P <0.05) ), The rest of the physiological growth, neurological reflex indicators and learning and memory function index differences were not statistically significant (P> 0.05). Conclusion There was no significant difference in the growth and neurological reflections of irradiated rats fed with irradiated diet and high-pressure diet, and the performance of passive fed rats was better than that fed high-pressure feed in passive memory.