论文部分内容阅读
目的检测不孕症患者体外受精-胚胎移植(IVF-ET)前种植窗期子宫内膜N-乙酰氨基葡萄糖-6-磺基转移酶(N-acetylglucosamine-6-O-sulfo trans ferase)的表达,比较受孕者和未受孕者的表达差异,探讨选择素与其配体相结合与胚泡的附植过程的关系及其发挥的作用。方法2004-07-2005-03南京医科大学第一附属医院生殖中心在进行IVF-ET治疗前,于黄体中期行宫腔镜检查的不孕症患者作为研究对象,受孕者14例作为研究组,未受孕者28例作为对照组,用反转录-聚合酶链反应(RT-PCR)方法检测两组患者种植窗期的表达水平。结果不孕症患者受孕组种植窗期子宫内膜N-乙酰氨基葡萄糖-6-磺基转移酶的mRNA的表达水平(灰度值)平均为(0.65±0.33),未受孕组种植窗期子宫内膜N-乙酰氨基葡萄糖-6-磺基转移酶的mRNA的表达水平(灰度值)平均为(0.41±0.36),其N-乙酰氨基葡萄糖-6-磺基转移酶的mRNA的表达水平显著低于受孕组,P<0.05。结论选择素与其配体相结合的机制可能参与了胚泡的附植过程,并可能是子宫内膜对胚胎接受能力的重要参数之一。
Objective To detect the expression of N-acetylglucosamine-6-O-sulfo trans ferase in the implantation window of infertile patients before in vitro fertilization-embryo transfer (IVF-ET) To compare the expression difference between pregnant women and non-pregnant women, and to explore the relationship between the combination of selectin and its ligands and the attachment of blastocysts and its role. Methods 2004-07-2005-03 First Affiliated Hospital of Nanjing Medical University Reproductive Center Before IVF-ET treatment, infertility patients who underwent hysteroscopy during the mid-luteal phase were selected as study subjects. Fourteen pregnant women were included as study group, Twenty-eight pregnant women served as the control group, and the expression level at the implantation window was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results The average expression level of gray level of endometrial N-acetylglucosamine-6-sulfotransferase in the pregnant women with infertility implantation group was (0.65 ± 0.33) The expression level (gray value) of mRNA of intimal N-acetylglucosamine-6-sulfotransferase was (0.41 ± 0.36) on average, and the expression level of mRNA of N-acetylglucosamine-6-sulfotransferase Significantly lower than the control group, P <0.05. Conclusion The mechanism of the combination of selectin and its ligand may be involved in the process of blastocyst attachment and may be one of the important parameters of endometrium receptivity to embryo.