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目的研究槲皮素对体外哺乳动物的细胞遗传毒性。方法采用80、40、20、10、5 mg/L5个剂量组的槲皮素在有或无代谢活化条件下处理体外培养的中国地鼠肺成纤维细胞(CHL)3 h后更换新鲜培养液,恢复生长21 h后收获细胞制片,观察槲皮素对哺乳动物细胞染色体的影响。采用200、100、50、25和12.5 mg/L 5个剂量组的槲皮素在有或无代谢活化条件下处理中国仓鼠肺成纤维细胞(V79)3 h后,经过7 d的次黄嘌呤鸟嘌呤磷酸核糖转移酶(HGPRT)突变基因表达和7 d突变基因选择后计数集落形成率并计突变频率,观察槲皮素对哺乳动物HGPRT基因位点的影响。结果在有或无代谢活化条件下槲皮素在浓度>10 mg/L均能够诱导CHL细胞染色体断裂和交换等,染色体细胞畸变率显著增加(P<0.01);而在有或无代谢活化与溶剂对照组相比较槲皮素各剂量组均未发生基因突变频率显著增加(P>0.05)。结论在体外试验条件下,槲皮素对哺乳动物细胞显示出明显致突变性,存在潜在的遗传毒性。
Objective To study the cytotoxicity of quercetin to mammalian cells in vitro. Methods Quercetin at doses of 80, 40, 20, 10, 5 mg / L was treated with or without metabolic activation for 3 h in vitro and cultured in vitro. After 21 h of growth recovery, cells were harvested and the effect of quercetin on the chromosome of mammalian cells was observed. Chinese hamster lung fibroblasts (V79) were treated with or without metabolic activation of quercetin at doses of 200, 100, 50, 25 and 12.5 mg / L for 3 h, and then hypoxanthine HGPRT gene expression and 7 d mutation gene selection counting colony formation rate and the frequency of mutation, to observe the effect of quercetin on mammalian HGPRT gene locus. Results With or without metabolic activation, quercetin could induce the chromosome breakage and exchange in CHL cells at a concentration> 10 mg / L, and the chromosome aberration rate was significantly increased (P <0.01). However, with or without metabolic activation and Compared with quercetin, the frequency of gene mutation in solvent control group was significantly increased (P> 0.05). Conclusions Quercetin shows obvious mutagenicity to mammalian cells under in vitro conditions, and there is potential genotoxicity.